Detailed view for Rv3670

Basic information

TDR Targets ID: 5937
Mycobacterium tuberculosis, Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase)
Source Database / ID:  Tuberculist 

pI: 10.1255 | Length (AA): 327 | MW (Da): 36334 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00561   alpha/beta hydrolase fold

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 322 1b6g (A) 5 309 19.00 0 1 1.10401 0.08
16 320 5ng7 (A) 6 288 30.00 0 1 1.25482 -0.49
24 96 2xt0 (A) 32 90 47.00 0.15 0.77 0.390442 0.62
51 322 3b12 (A) 22 291 26.00 0 1 1.1639 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_130074)

Species Accession Gene Product
Candida albicans CaO19.6709   has match to alpha/beta hydrolase fold
Candida albicans CaO19.14001   has match to alpha/beta hydrolase fold
Caenorhabditis elegans CELE_K07C5.5   Protein CEEH-2, isoform B
Caenorhabditis elegans CELE_K02F3.6   Protein CEEH-1
Homo sapiens ENSG00000172031   epoxide hydrolase 4
Mycobacterium leprae ML2297   POSSIBLE EPOXIDE HYDROLASE EPHE (EPOXIDE HYDRATASE) (ARENE-OXIDE HYDRATASE)
Mus musculus ENSMUSG00000033805   epoxide hydrolase 4
Mycobacterium tuberculosis Rv3670   Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase)
Mycobacterium ulcerans MUL_4244   epoxide hydrolase EphE
Schmidtea mediterranea mk4.002527.01  

Essentiality

No essentiality data collected for this gene and/or its orthologs.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
ChEMBL23 References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Renilla reniformis Renilla-luciferin 2-monooxygenase 311 aa 21.1% 284 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0228 1 0.5
0.022 0.9801 0.9774
0.0193 0.6263 1
0.0193 0.6263 1

Assayability

Assay information

  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis recombinant EphE expressed in Escherichia coli BL21 using CMNPC as substrate at 10 nM after 30 mins by fluorescent assay relative to control
  • ChEMBL
  • Inhibition of Mycobacterium tuberculosis recombinant EphE expressed in Escherichia coli BL21 using CMNPC as substrate after 30 mins by fluorescent assay relative to control

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3670 (Mycobacterium tuberculosis), Possible epoxide hydrolase EphE (epoxide hydratase) (arene-oxide hydratase)
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