Detailed view for CaO19.2929

Basic information

TDR Targets ID: 648942
Candida albicans, beta-1,3-glucan synthase similar to S. cerevisiae GSC2 (YGR032W) and FKS1 (YLR342W) beta-1,3-glucan synthase
Source Database / ID:  KEGG  

pI: 8.0111 | Length (AA): 1897 | MW (Da): 218058 | Paralog Number: 7

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 18

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02364   1,3-beta-glucan synthase component
PF14288   1,3-beta-glucan synthase subunit FKS1, domain-1

Gene Ontology

Mouse over links to read term descriptions.
GO:0003843   1,3-beta-glucan synthase activity  
GO:0000148   1,3-beta-glucan synthase complex  
GO:0016020   membrane  

Metabolic Pathways

Starch and sucrose metabolism (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127693)

Species Accession Gene Product
Arabidopsis thaliana AT4G04970   glucan synthase-like 1
Arabidopsis thaliana AT2G31960   glucan synthase-like 3
Arabidopsis thaliana AT1G05570   callose synthase 1
Arabidopsis thaliana AT3G59100   putative callose synthase 6
Arabidopsis thaliana AT2G13680   callose synthase 5
Arabidopsis thaliana AT2G36850   glucan synthase-like 8
Arabidopsis thaliana AT1G06490   callose synthase 7
Arabidopsis thaliana AT5G13000   callose synthase 3
Arabidopsis thaliana AT3G14570   glucan synthase-like 4
Arabidopsis thaliana AT4G03550   callose synthase 12
Arabidopsis thaliana AT3G07160   callose synthase 9
Arabidopsis thaliana AT5G36870   callose synthase 4
Candida albicans CaO19.3269   N terminus of glucan synthase
Candida albicans CaO19.10780   C terminus of glucan synthase
Candida albicans CaO19.3270   C terminus of glucan synthase
Candida albicans CaO19.10031   similar to S. cerevisiae FKS3 (YMR306W) 1,3-beta glucan synthase
Candida albicans CaO19.10779   N terminus of glucan synthase
Candida albicans CaO19.10446   beta-1,3-glucan synthase similar to S. cerevisiae GSC2 (YGR032W) and FKS1 (YLR342W) beta-1,3-glucan synthase
Candida albicans CaO19.2495   glucan synthase
Candida albicans CaO19.2929   beta-1,3-glucan synthase similar to S. cerevisiae GSC2 (YGR032W) and FKS1 (YLR342W) beta-1,3-glucan synthase
Neospora caninum NCLIV_067880   hypothetical protein
Oryza sativa 4325900   Os01g0672500
Oryza sativa 4331485   Os03g0128100
Oryza sativa 4327809   Os01g0533500
Oryza sativa 9269677   Os03g0128200
Oryza sativa 4327807   Os01g0532900
Oryza sativa 4339903   Os06g0112800
Oryza sativa 4325131   Os01g0754200
Oryza sativa 4340315   Os06g0182300
Oryza sativa 9271845   Os06g0728766
Oryza sativa 9271759   Os01g0533800
Oryza sativa 4331423   Os03g0119500
Oryza sativa 4331259   Os02g0832400
Oryza sativa 6450172   hypothetical protein
Oryza sativa 4331260   Os02g0832500
Oryza sativa 4342136   Os06g0728800
Saccharomyces cerevisiae YMR306W   putative 1,3-beta-D-glucan synthase
Saccharomyces cerevisiae YLR342W   1,3-beta-D-glucan synthase
Saccharomyces cerevisiae YGR032W   1,3-beta-glucan synthase GSC2
Toxoplasma gondii TGME49_278110   1,3-beta-glucan synthase component protein

Essentiality

CaO19.2929 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_278110 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

196 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Pichia kudriavzevii 1,3-beta-D-glucan synthase subunit Compounds References
Candida albicans Glucan synthase Compounds References
Candida glabrata 1,3-beta-glucan synthase Compounds References
Candida albicans C terminus of glucan synthase Compounds References
Aspergillus 1,3-beta-glucan synthase Compounds References
Saccharomyces cerevisiae 1,3-beta-glucan synthase GSC2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Tested for inhibition of Beta-1,3-glucan synthase using a crude membrane of Candida albicans (MY 1208). ChEMBL
  • Tested for inhibition of Beta-1,3-glucan synthase using a crude membrane of Candida albicans (MY 1208).
  • In vitro inhibition of glucan synthase obtained from C. albicans at a concentration of 10 ug/mL. ChEMBL
  • In vitro inhibition of glucan synthase obtained from Candida albicans at a concentration of 10 ug/mL.
  • In vitro inhibitory activity against Beta glucan synthase ChEMBL
  • In vitro inhibitory activity against Beta glucan synthase
  • Inhibition of 1,3-beta-D-glucan synthase (GS) from candida albicans ChEMBL
  • Inhibition of 1,3-beta-D-glucan synthase (GS) from candida albicans
  • ChEMBL
  • BindingDB_Patents: Glucan Synthase Assay. The glucan synthase assay follows the incorporation of tritiated UDP-glucose into acid insoluble ß-glucan as catalysed by the glucan synthase activity present in membrane preparations from Candida albicans and Aspergillus fumigatus. UDP-[3H]-glucose (ca 0.01 µCi) is added to 100 µl assay buffer (50 mM Tris-Cl, pH8.0, 8% glycerol, 1 mM EDTA, 1.5 mM KF, 1 mM DTT, 20 µM GTP¿S, 600 µM UDP-glucose) and the reaction initiated by the addition of 2.5 µl of enzyme preparation. After incubation at 30°C. for 120 minutes, 10 µl of 30 mg/ml BSA is added with mixing and the reaction is terminated with 110 µl ice-cold 20% TCA. The precipitate is collected on a GF/B filter plate and washed three times with 200 µl water. Once dry, 200 µl of Microscint20 is added to each well and the plate is read on a Top-count scintillation counter.
  • ChEMBL
  • Inhibition of Candida albicans ATCC 90028 glucan synthase at monophasic kinetics
  • ChEMBL
  • Inhibition of Candida albicans 1,3 beta-D-glucan synthase
  • ChEMBL
  • Inhibition of Candida albicans BWP17 1,3-beta-D-glucan synthase
  • ChEMBL
  • Inhibition of Candida albicans BWP17 1,3-beta-glucan synthase
  • ChEMBL
  • Inhibition of glucan synthase in Candida albicans BWP17 membrane
  • ChEMBL
  • Inhibition of 1,3-beta-D-glucan synthase in Candida albicans BWP17 membranes
  • ChEMBL
  • Inhibition of wild type Candida albicans 1,3-beta-D-glucan synthase using UDP[6,3H]-glucose as substrate after 60 mins by liquid scintillation counting analysis
  • ChEMBL
  • Inhibition of wild type Candida albicans MY1055 1,3-beta-D-glucan synthase using UDP[6,3H]-glucose as substrate after 60 mins by liquid scintillation counting analysis
  • ChEMBL
  • Displacement of UDP-[3H]glucose from beta-1,3-glucan synthase in Candida albicans MY1055 microsomal membranes incubated for 2 hrs

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier CaO19.2929 (Candida albicans), beta-1,3-glucan synthase similar to S. cerevisiae GSC2 (YGR032W) and FKS1 (YLR342W) beta-1,3-glucan synthase
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