Detailed view for Rv3109

Basic information

TDR Targets ID: 6496
Mycobacterium tuberculosis, Probable molybdenum cofactor biosynthesis protein A MoaA1
Source Database / ID:  Tuberculist 

pI: 8.1767 | Length (AA): 359 | MW (Da): 39960 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04055   Radical SAM superfamily
PF06463   Molybdenum Cofactor Synthesis C
PF13353   4Fe-4S single cluster domain
PF13394  

Gene Ontology

Mouse over links to read term descriptions.
GO:0051539   4 iron, 4 sulfur cluster binding  
GO:0019008   molybdopterin synthase complex  
GO:0046872   metal ion binding  
GO:0003824   catalytic activity  
GO:0051536   iron-sulfur cluster binding  
GO:0008152   metabolic process  
GO:0006777   Mo-molybdopterin cofactor biosynthetic process  

Metabolic Pathways

Folate biosynthesis (KEGG)
Global map (KEGG)
Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 335 1tv8 (A) 4 324 37.00 0 1 1.36668 -0.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_128145)

Species Accession Gene Product
Arabidopsis thaliana AT2G31955   cofactor of nitrate reductase and xanthine dehydrogenase 2
Caenorhabditis elegans CELE_F49H6.5   Protein F49H6.5
Caenorhabditis elegans CELE_F49E2.1   Protein F49E2.1, isoform A
Dictyostelium discoideum DDB_G0285137   molybdenum cofactor synthesis 1
Drosophila melanogaster Dmel_CG33048   Molybdenum cofactor synthesis 1 ortholog
Escherichia coli b0781   molybdopterin biosynthesis protein A
Homo sapiens ENSG00000124615   molybdenum cofactor synthesis 1
Mus musculus ENSMUSG00000064120   molybdenum cofactor synthesis 1
Mycobacterium tuberculosis Rv3109   Probable molybdenum cofactor biosynthesis protein A MoaA1
Mycobacterium tuberculosis Rv0869c   Probable molybdenum cofactor biosynthesis protein A2 MoaA2
Mycobacterium ulcerans MUL_0281   molybdenum cofactor biosynthesis protein A
Neospora caninum NCLIV_013520   molybdopterin synthase, putative
Oryza sativa 4352298   Os12g0507000
Oryza sativa 4328930   Os02g0258900
Schistosoma japonicum Sjp_0091650   ko:K03639 molybdenum cofactor biosynthesis protein A, putative
Schistosoma japonicum Sjp_0134950   ko:K03639 molybdenum cofactor biosynthesis protein A, putative
Schistosoma mansoni Smp_171270   molybdopterin cofactor synthesis protein A
Schmidtea mediterranea mk4.000527.12   Molybdenum cofactor biosynthesis protein 1
Schmidtea mediterranea mk4.000527.13   Molybdenum cofactor biosynthesis protein 1
Toxoplasma gondii TGME49_213780   radical SAM domain-containing protein

Essentiality

Rv3109 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3162 this record Mycobacterium tuberculosis essential nmpdr
mtu885 Mycobacterium tuberculosis non-essential nmpdr
b0781 Escherichia coli non-essential goodall
CELE_F49E2.1 Caenorhabditis elegans adult lethal wormbase
CELE_F49E2.1 Caenorhabditis elegans slow growth wormbase
CELE_F49H6.5 Caenorhabditis elegans adult lethal wormbase
CELE_F49H6.5 Caenorhabditis elegans slow growth wormbase
TGME49_213780 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3109 (Mycobacterium tuberculosis), Probable molybdenum cofactor biosynthesis protein A MoaA1
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