Detailed view for Rv0319

Basic information

TDR Targets ID: 6655
Mycobacterium tuberculosis, Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase)
Source Database / ID:  Tuberculist 

pI: 6.4816 | Length (AA): 222 | MW (Da): 23193 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01470   Pyroglutamyl peptidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016920   pyroglutamyl-peptidase activity  
GO:0005829   cytosol  
GO:0008234   cysteine-type peptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 216 1a2z (A) 1 216 34.00 0 1 1.46787 -1.01
1 211 4hps (A) 1 206 41.00 0 1 1.54565 -1.12
3 208 1x10 (A) 2 206 37.00 0 1 1.49813 -1.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_128512)

Species Accession Gene Product
Arabidopsis thaliana AT1G23440   peptidase C15, pyroglutamyl peptidase I-like protein
Arabidopsis thaliana AT1G56700   peptidase C15, pyroglutamyl peptidase I-like protein
Brugia malayi Bm1_11435   hypothetical protein
Caenorhabditis elegans CELE_M04C9.3   Protein M04C9.3, isoform B
Caenorhabditis elegans CELE_M04C9.1   Protein M04C9.1, isoform B
Dictyostelium discoideum DDB_G0267498   hypothetical protein
Drosophila melanogaster Dmel_CG32147   CG32147 gene product from transcript CG32147-RA
Echinococcus granulosus EgrG_000217600   Peptidase C15 pyroglutamyl peptidase I
Echinococcus multilocularis EmuJ_000217600   Peptidase C15, pyroglutamyl peptidase I
Homo sapiens ENSG00000183571   pyroglutamyl-peptidase I-like
Homo sapiens ENSG00000130517   pyroglutamyl-peptidase I
Loa Loa (eye worm) LOAG_10815   hypothetical protein
Mus musculus ensembl-mmu:ENSMUSG00000056204   pyroglutamyl-peptidase I
Mus musculus ENSMUSG00000030553   pyroglutamyl-peptidase I-like
Mycobacterium tuberculosis Rv0319   Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase)
Neospora caninum NCLIV_037920   Pyrrolidone-carboxylate peptidase (EC 3.4.19.3), related
Oryza sativa 4332921   Os03g0367900
Oryza sativa 4344054   Os07g0639600
Oryza sativa 4346976   Os09g0396300
Schistosoma japonicum Sjp_0059190   ko:K01304 pyroglutamyl-peptidase [EC3.4.19.3], putative
Schistosoma mansoni Smp_140810   pyroglutamyl-peptidase I (C15 family)
Toxoplasma gondii TGME49_268280   'chromo' (CHRromatin Organization MOdifier) domain-containing protein
Trichomonas vaginalis TVAG_108550   Clan CF, family C15, pyroglutamyl peptidase I-like cysteine peptidase

Essentiality

Rv0319 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu322 this record Mycobacterium tuberculosis non-essential nmpdr
TGME49_268280 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Pyroglutamate Aminopeptidase (3.4.19.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0319 (Mycobacterium tuberculosis), Probable pyrrolidone-carboxylate peptidase Pcp (5-oxoprolyl-peptidase) (pyroglutamyl-peptidase I) (PGP-I) (pyrase)
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