Detailed view for Rv0726c

Basic information

TDR Targets ID: 7534
Mycobacterium tuberculosis, Possible S-adenosylmethionine-dependent methyltransferase
Source Database / ID:  Tuberculist 

pI: 5.4503 | Length (AA): 367 | MW (Da): 40876 | Paralog Number: 8

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04072   Leucine carboxyl methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0032259   methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
14 312 2ckd (A) 8 309 42.00 0 1 1.39361 -0.87
14 317 2ckd (A) 8 309 46.00 0 1 1.40584 -0.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_135170)

Species Accession Gene Product
Arabidopsis thaliana AT5G42760   leucine carboxyl methyltransferase
Dictyostelium discoideum DDB_G0285065   hypothetical protein
Mycobacterium leprae ML2640c   conserved hypothetical protein
Mycobacterium tuberculosis Rv1729c   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv0146   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv0145   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv3787c   Conserved hypothetical protein
Mycobacterium tuberculosis Rv0725c   Conserved hypothetical protein
Mycobacterium tuberculosis Rv0726c   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv0731c   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv3399   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium tuberculosis Rv0830   Possible S-adenosylmethionine-dependent methyltransferase
Mycobacterium ulcerans MUL_0818   O-methyltransferase
Mycobacterium ulcerans MUL_2766   O-methyltransferase
Mycobacterium ulcerans MUL_0450   O-methyltransferase
Mycobacterium ulcerans MUL_4430   hypothetical protein
Mycobacterium ulcerans MUL_4763   hypothetical protein
Mycobacterium ulcerans MUL_4761   O-methyltransferase
Mycobacterium ulcerans MUL_0817   O-methyltransferase
Mycobacterium ulcerans MUL_4762   O-methyltransferase
Mycobacterium ulcerans MUL_0816   O-methyltransferase
Oryza sativa 4334283   Os03g0775000

Essentiality

Rv0726c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu147 Mycobacterium tuberculosis non-essential nmpdr
mtu744 Mycobacterium tuberculosis non-essential nmpdr
mtu846 Mycobacterium tuberculosis non-essential nmpdr
mtu3460 Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0726c (Mycobacterium tuberculosis), Possible S-adenosylmethionine-dependent methyltransferase
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