Detailed view for EHI_189970

Basic information

TDR Targets ID: 798020
Entamoeba histolytica, amino acid permease, putative
Source Database / ID:  AmoebaDB  

pI: 6.5963 | Length (AA): 495 | MW (Da): 55724 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 12

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13520   Amino acid permease

Gene Ontology

Mouse over links to read term descriptions.
GO:0022857   transmembrane transporter activity  
GO:0016020   membrane  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 485 3gia (A) 5 424 17.00 0 0.11 1.01387 -0.44
43 466 3l1l (A) 17 423 20.00 0 0.84 1.06727 -0.26
52 129 4hzu (S) 60 149 40.00 0.32 0.07 0.288076 0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126658)

Species Accession Gene Product
Brugia malayi Bm1_48700   Amino acid permease family protein
Brugia malayi Bm1_35135   Amino acid permease family protein
Candida albicans CaO19.7953   potential amino acid permease similar to S. cerevisiae MUP3 (YHL036W) very low affinity methionine permease
Candida albicans CaO19.321   potential amino acid permease similar to S. cerevisiae MUP3 (YHL036W) very low affinity methionine permease
Caenorhabditis elegans CELE_F27C8.1   Protein AAT-1
Caenorhabditis elegans CELE_F52H2.2   Protein AAT-3
Caenorhabditis elegans CELE_F07C3.7   Protein AAT-2
Drosophila melanogaster Dmel_CG9413   CG9413 gene product from transcript CG9413-RD
Drosophila melanogaster Dmel_CG1607   CG1607 gene product from transcript CG1607-RA
Drosophila melanogaster Dmel_CG12317   CG12317 gene product from transcript CG12317-RE
Drosophila melanogaster Dmel_CG6070   genderblind
Drosophila melanogaster Dmel_CG3297   minidiscs
Escherichia coli b4115   arginine:agmatine antiporter
Escherichia coli b3370   putative fructoselysine transporter
Escherichia coli b0692   putrescine/proton symporter: putrescine/ornithine antiporter
Echinococcus granulosus EgrG_001011500   cationic amino acid transporter
Echinococcus granulosus EgrG_000914000   5'partial|cationic amino acid transporter
Echinococcus granulosus EgrG_000829200   yl amino acid transporter 2
Echinococcus granulosus EgrG_000913600   Large neutral amino acids transporter small
Echinococcus granulosus EgrG_000913800   cationic amino acid transporter
Echinococcus granulosus EgrG_000914200   amino acids transporter
Entamoeba histolytica EHI_189970   amino acid permease, putative
Echinococcus multilocularis EmuJ_000002800   amino acid permease
Echinococcus multilocularis EmuJ_000829200   y+l amino acid transporter 2
Echinococcus multilocularis EmuJ_000914200   amino acids transporter
Echinococcus multilocularis EmuJ_000913800   cationic amino acid transporter
Echinococcus multilocularis EmuJ_000914100   cationic amino acid transporter
Echinococcus multilocularis EmuJ_000913900   Large neutral amino acids transporter small
Echinococcus multilocularis EmuJ_000913600   Large neutral amino acids transporter small
Echinococcus multilocularis EmuJ_001011500   cationic amino acid transporter
Echinococcus multilocularis EmuJ_000914300   cationic amino acid transporter
Homo sapiens ENSG00000092068   solute carrier family 7 (amino acid transporter light chain, L system), member 8
Homo sapiens ENSG00000155465   solute carrier family 7 (amino acid transporter light chain, y+L system), member 7
Homo sapiens ENSG00000103257   solute carrier family 7 (amino acid transporter light chain, L system), member 5
Homo sapiens ENSG00000103064   solute carrier family 7 (amino acid transporter light chain, y+L system), member 6
Homo sapiens ENSG00000021488   solute carrier family 7 (amino acid transporter light chain, bo,+ system), member 9
Homo sapiens ENSG00000130876   solute carrier family 7 (neutral amino acid transporter light chain, asc system), member 10
Loa Loa (eye worm) LOAG_02588   amino acid permease
Loa Loa (eye worm) LOAG_12582   amino acid permease
Mus musculus ENSMUSG00000031904   solute carrier family 7 (cationic amino acid transporter, y+ system), member 6
Mus musculus ENSMUSG00000030492   solute carrier family 7 (cationic amino acid transporter, y+ system), member 9
Mus musculus ENSMUSG00000040010   solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
Mus musculus ENSMUSG00000030495   solute carrier family 7 (cationic amino acid transporter, y+ system), member 10
Mus musculus ENSMUSG00000022180   solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Mus musculus ENSMUSG00000000958   solute carrier family 7 (cationic amino acid transporter, y+ system), member 7
Onchocerca volvulus OVOC5775  
Saccharomyces cerevisiae YHL036W   Mup3p
Schistosoma japonicum Sjp_0018300   Y+L amino acid transporter 1, putative
Schistosoma japonicum Sjp_0208770   Cystine/glutamate transporter, putative
Schistosoma japonicum Sjp_0214560   ko:K03450 L-amino acid transporter, LAT family, putative
Schistosoma japonicum Sjp_0118650   IPR002293,Amino acid/polyamine transporter I,domain-containing
Schistosoma mansoni Smp_150990   cationic amino acid transporter
Schistosoma mansoni Smp_054010   cationic amino acid transporter
Schistosoma mansoni Smp_083990.2   cationic amino acid transporter
Schistosoma mansoni Smp_083990.1   cationic amino acid transporter
Schmidtea mediterranea mk4.027108.06  
Schmidtea mediterranea mk4.038743.00  
Schmidtea mediterranea mk4.011301.01   Cystine/glutamate transporter
Schmidtea mediterranea mk4.002524.01   Cystine/glutamate transporter
Schmidtea mediterranea mk4.006181.00  
Schmidtea mediterranea mk4.010961.02  
Schmidtea mediterranea mk4.000111.06  
Schmidtea mediterranea mk4.009375.00   Cystine/glutamate transporter
Schmidtea mediterranea mk4.002524.00   Cystine/glutamate transporter
Schmidtea mediterranea mk4.000467.01   Cystine/glutamate transporter
Schmidtea mediterranea mk4.011528.00  
Schmidtea mediterranea mk4.021798.00   Cystine/glutamate transporter
Schmidtea mediterranea mk4.031652.00   Cystine/glutamate transporter

Essentiality

EHI_189970 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0692 Escherichia coli non-essential goodall
b3370 Escherichia coli non-essential goodall
b4115 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens solute carrier family 7 (amino acid transporter light chain, L system), member 5 Compounds References
Rattus norvegicus L-type amino acid transporter 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0104 0.5201 0.5
0.0104 0.5201 0.5
0.0104 0.5201 0.5
0.0026 1 0.5
0.011 0.5201 0.5
0.0026 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier EHI_189970 (Entamoeba histolytica), amino acid permease, putative
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