Detailed view for EHI_093330

Basic information

TDR Targets ID: 798280
Entamoeba histolytica, Helix-turn-helix protein, putative
Source Database / ID:  AmoebaDB  

pI: 10.545 | Length (AA): 125 | MW (Da): 13936 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01381   Helix-turn-helix

Gene Ontology

Mouse over links to read term descriptions.
GO:0043565   sequence-specific DNA binding  
GO:0003677   DNA binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 123 2jvl (A) 49 142 38.00 0.000000000013 1 1.2507 -0.64
53 124 1x57 (A) 1 72 58.00 0 1 1.4127 -1.73
60 105 1zzc (A) 5 50 24.00 0 0.51 0.8257 -1.83
69 98 2w48 (A) 12 41 23.00 0.9 0.74 0.7587 -2.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127833)

Species Accession Gene Product
Arabidopsis thaliana AT2G42680   multiprotein bridging factor 1A
Arabidopsis thaliana AT3G58680   multiprotein-bridging factor 1b
Arabidopsis thaliana AT3G24500   multiprotein-bridging factor 1c
Babesia bovis BBOV_I000130   multiprotein bridging factor type 1
Brugia malayi Bm1_39920   homologous to Bombyx mori multiprotein bridging factor, putative
Candida albicans CaO19.10804   multiprotein bridging factor
Candida albicans CaO19.3294   multiprotein bridging factor
Caenorhabditis elegans CELE_H21P03.1   Protein MBF-1
Cryptosporidium hominis Chro.30426   multiprotein bridging factor type 1
Cryptosporidium parvum cgd3_3750   multiprotein bridging factor type 1 like transcriptional co-activator
Dictyostelium discoideum DDB_G0273061   hypothetical protein
Dictyostelium discoideum DDB_G0273775   hypothetical protein
Drosophila melanogaster Dmel_CG4143   multiprotein bridging factor 1
Echinococcus granulosus EgrG_000845500   endothelial differentiation factor
Entamoeba histolytica EHI_093330   Helix-turn-helix protein, putative
Echinococcus multilocularis EmuJ_000845500   endothelial differentiation factor
Homo sapiens ENSG00000107223   endothelial differentiation-related factor 1
Leishmania braziliensis LbrM.19.0500   hypothetical protein, conserved
Leishmania donovani LdBPK_190180.1   multiprotein-bridging factor 1, putative
Leishmania infantum LinJ.19.0180   hypothetical protein, conserved
Leishmania major LmjF.19.0190   hypothetical protein, conserved
Leishmania mexicana LmxM.19.0190   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05345   MBF-1 protein
Mus musculus ENSMUSG00000015092   endothelial differentiation-related factor 1
Mus musculus 665181   predicted gene 11964
Neospora caninum NCLIV_067470   hypothetical protein
Oryza sativa 4345388   Os08g0366100
Oryza sativa 4341420   Os06g0592500
Plasmodium berghei PBANKA_0920000   multiprotein-bridging factor 1, putative
Plasmodium falciparum PF3D7_1128200   multiprotein-bridging factor 1, putative
Plasmodium knowlesi PKNH_0926300   multiprotein-bridging factor 1, putative
Plasmodium vivax PVX_092030   multiprotein bridging factor type 1, putative
Plasmodium yoelii PY05916   multiprotein bridging factor type 1
Saccharomyces cerevisiae YOR298C-A   Mbf1p
Schistosoma japonicum Sjp_0038760   ko:K03627 putative transcription factor, putative
Schistosoma mansoni Smp_140680.1   endothelial differentiation-related factor 1
Schistosoma mansoni Smp_140680.2   endothelial differentiation-related factor 1
Schmidtea mediterranea mk4.025256.00   Endothelial differentiation-related factor 1
Schmidtea mediterranea mk4.001374.01   Endothelial differentiation-related factor 1
Schmidtea mediterranea mk4.012724.00   Endothelial differentiation-related factor 1
Trypanosoma brucei gambiense Tbg972.10.17980   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.14810   multiprotein-bridging factor 1, putative
Trypanosoma congolense TcIL3000_10_12680   multiprotein-bridging factor 1, putative
Trypanosoma cruzi TcCLB.511289.59   multiprotein-bridging factor 1, putative
Trypanosoma cruzi TcCLB.506211.170   multiprotein-bridging factor 1, putative
Toxoplasma gondii TGME49_278530   multiprotein bridging factor type 1 family transcriptional co-activator, putative
Theileria parva TP03_0858   multiprotein bridging factor type 1, putative
Trichomonas vaginalis TVAG_303670   conserved hypothetical protein

Essentiality

EHI_093330 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.14810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14810 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14810 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_278530 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier EHI_093330 (Entamoeba histolytica), Helix-turn-helix protein, putative
Title for this comment
Comment