Detailed view for EHI_006770

Basic information

TDR Targets ID: 798293
Entamoeba histolytica, hypothetical protein, conserved
Source Database / ID:  AmoebaDB  

pI: 6.6896 | Length (AA): 231 | MW (Da): 26407 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01467   Cytidylyltransferase-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 57 4d0n (B) 2292 2340 33.00 0.87 0.11 0.421237 0.61
80 210 3do8 (A) 3 133 28.00 0.0000034 0.94 0.9369 -0.54
80 224 3do8 (A) 3 140 28.00 0 0.99 0.887306 -0.03
82 223 1o6b (A) 7 135 14.00 0 0.13 0.629319 0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128320)

Species Accession Gene Product
Arabidopsis thaliana AT2G18250   phosphopantetheine adenylyltransferase
Babesia bovis BBOV_III002660   hypothetical protein
Brugia malayi Bm1_41820   dephospho-CoA kinase family protein
Candida albicans CaO19.1776   similar to S. cerevisiae YGR277C
Candida albicans CaO19.9343   similar to S. cerevisiae YGR277C
Caenorhabditis elegans CELE_Y65B4A.8   Protein Y65B4A.8
Dictyostelium discoideum DDB_G0288465   hypothetical protein
Drosophila melanogaster Dmel_CG10575   Bifunctional Phosphopantetheine adenylyltransferase - Dephospho-CoA kinase
Echinococcus granulosus EgrG_000575100   dephospho coenzyme A related kinases
Entamoeba histolytica EHI_155780   dephospho-CoA kinase, putative
Entamoeba histolytica EHI_006680   phosphopantetheine adenylyltransferase, putative
Entamoeba histolytica EHI_006770   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000575100   dephospho coenzyme A related kinases
Echinococcus multilocularis EmuJ_000009100   bifunctional coenzyme A synthase
Homo sapiens ENSG00000068120   CoA synthase
Leishmania braziliensis LbrM.32.2280   hypothetical protein, conserved
Leishmania donovani LdBPK_322200.1   cytidylyltransferase, putative
Leishmania infantum LinJ.32.2200   hypothetical protein, conserved
Leishmania major LmjF.32.2070   hypothetical protein, conserved
Leishmania mexicana LmxM.31.2070   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08818   hypothetical protein
Mus musculus ENSMUSG00000001755   Coenzyme A synthase
Oryza sativa 4342557   Os07g0179400
Oryza sativa 9271371   Os02g0134000
Saccharomyces cerevisiae YGR277C   putative pantetheine-phosphate adenylyltransferase
Schistosoma japonicum Sjp_0042770   ko:K00859 dephospho-CoA kinase [EC2.7.1.24], putative
Schistosoma mansoni Smp_149950   dephospho-CoA related kinases
Schmidtea mediterranea mk4.023191.00  
Schmidtea mediterranea mk4.024021.00  
Schmidtea mediterranea mk4.006944.02   Bifunctional coenzyme A synthase
Schmidtea mediterranea mk4.033371.01  
Trypanosoma brucei gambiense Tbg972.11.17150   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.15270   phosphopantetheine adenylyltransferase, putative
Trypanosoma congolense TcIL3000.11.15410   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506425.140   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511711.50   hypothetical protein, conserved
Trichomonas vaginalis TVAG_463570   Phosphopantetheine adenylyltransferase, putative

Essentiality

EHI_006770 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6920 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6920 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6920 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.6920 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y65B4A.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y65B4A.8 Caenorhabditis elegans larval arrest wormbase
CELE_Y65B4A.8 Caenorhabditis elegans sterile wormbase
YGR277C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_006770 (Entamoeba histolytica), hypothetical protein, conserved
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