Detailed view for EHI_025350

Basic information

TDR Targets ID: 798645
Entamoeba histolytica, importin alpha, putative
Source Database / ID:  AmoebaDB  

pI: 4.7341 | Length (AA): 478 | MW (Da): 55282 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 475 1wa5 (B) 15 513 19.00 0 1 1.26963 -1.06
9 155 5mfg (D) 12 159 20.00 0.0048 0.44 0.680231 -1.42
62 159 3nmw (A) 631 732 24.00 0.99 1 0.673921 -1.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_126761)

Species Accession Gene Product
Arabidopsis thaliana AT1G02690   importin alpha isoform 6
Arabidopsis thaliana AT1G09270   importin alpha isoform 4
Arabidopsis thaliana AT4G16143   importin alpha isoform 2
Arabidopsis thaliana AT5G49310   importin alpha isoform 5
Arabidopsis thaliana AT3G06720   Importin subunit alpha-1
Arabidopsis thaliana AT4G02150   Importin alpha 3
Babesia bovis BBOV_III009880   armadillo/beta-catenin-like repeat domain containing protein
Brugia malayi Bm1_32450   Importin alpha-3 subunit
Candida albicans CaO19.5682   Karyopherin-alpha or importin
Caenorhabditis elegans CELE_F32E10.4   Protein IMA-3
Cryptosporidium hominis Chro.80378   importin alpha
Cryptosporidium parvum cgd8_3260   importin alpha subunit
Dictyostelium discoideum DDB_G0272318   hypothetical protein
Drosophila melanogaster Dmel_CG8548   karyopherin alpha1
Drosophila melanogaster Dmel_CG9423   karyopherin alpha3
Echinococcus granulosus EgrG_000479600   importin subunit alpha 3
Entamoeba histolytica EHI_148070   importin alpha, putative
Entamoeba histolytica EHI_179940   hypothetical protein
Entamoeba histolytica EHI_025350   importin alpha, putative
Echinococcus multilocularis EmuJ_000479600   importin subunit alpha 3
Homo sapiens ENSG00000025800   karyopherin alpha 6 (importin alpha 7)
Homo sapiens ENSG00000114030   karyopherin alpha 1 (importin alpha 5)
Homo sapiens ENSG00000102753   karyopherin alpha 3 (importin alpha 4)
Homo sapiens ENSG00000186432   karyopherin alpha 4 (importin alpha 3)
Homo sapiens ENSG00000196911   karyopherin alpha 5 (importin alpha 6)
Leishmania braziliensis LbrM.30.1240   importin alpha, putative
Leishmania donovani LdBPK_301180.1   importin alpha, putative
Leishmania infantum LinJ.30.1180   importin alpha, putative
Leishmania major LmjF.30.1120   importin alpha, putative
Leishmania mexicana LmxM.29.1120   importin alpha, putative
Loa Loa (eye worm) LOAG_14865   hypothetical protein
Loa Loa (eye worm) LOAG_01346   importin alpha-3 subunit
Mus musculus ENSMUSG00000022905   karyopherin (importin) alpha 1
Mus musculus ENSMUSG00000027782   karyopherin (importin) alpha 4
Mus musculus ENSMUSG00000021929   karyopherin (importin) alpha 3
Mus musculus ENSMUSG00000003731   karyopherin (importin) alpha 6
Neospora caninum NCLIV_007390   MGC78841 protein, related
Oryza sativa 4327117   Os01g0253300
Oryza sativa 4326488   Os01g0343200
Oryza sativa 4337853   Os05g0155500
Plasmodium berghei PBANKA_1425100   karyopherin alpha, putative
Plasmodium falciparum PF3D7_0812400   karyopherin alpha
Plasmodium knowlesi PKNH_1428200   karyopherin alpha, putative
Plasmodium vivax PVX_123150   karyopherin alpha, putative
Plasmodium yoelii PY01795   putative impotin alpha 1b
Saccharomyces cerevisiae YNL189W   karyopherin alpha
Schistosoma japonicum Sjp_0070960   Importin subunit alpha-3, putative
Schistosoma mansoni Smp_051210.1   importin alpha 34
Schistosoma mansoni Smp_051210.3   importin alpha 34
Schmidtea mediterranea mk4.007993.00  
Schmidtea mediterranea mk4.003549.01  
Schmidtea mediterranea mk4.022525.00   Importin subunit alpha-3
Schmidtea mediterranea mk4.031324.00   Importin subunit alpha-3
Schmidtea mediterranea mk4.007005.00   Importin subunit alpha-3
Schmidtea mediterranea mk4.003829.03   Importin subunit alpha-3
Trypanosoma brucei gambiense Tbg972.6.2400   importin alpha subunit, putative
Trypanosoma brucei Tb927.6.2640   importin alpha subunit, putative
Trypanosoma congolense TcIL3000_6_2120   importin alpha subunit, putative
Trypanosoma cruzi TcCLB.509965.110   importin alpha, putative
Trypanosoma cruzi TcCLB.509057.20   importin alpha, putative
Toxoplasma gondii TGME49_252290   importin alpha, putative
Theileria parva TP04_0908   importin alpha, putative
Trichomonas vaginalis TVAG_235230   conserved hypothetical protein

Essentiality

EHI_025350 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2640 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2640 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2640 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.2640 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F32E10.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F32E10.4 Caenorhabditis elegans larval arrest wormbase
CELE_F32E10.4 Caenorhabditis elegans slow growth wormbase
CELE_F32E10.4 Caenorhabditis elegans sterile wormbase
YNL189W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1425100 Plasmodium berghei Essential plasmo
TGME49_252290 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_025350 (Entamoeba histolytica), importin alpha, putative
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