Detailed view for EHI_151190

Basic information

TDR Targets ID: 799564
Entamoeba histolytica, DEAD/DEAH box helicase, putative
Source Database / ID:  AmoebaDB  

pI: 8.5553 | Length (AA): 499 | MW (Da): 58005 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain
PF13959   Domain of unknown function (DUF4217)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 215 2pl3 (A) 68 276 37.00 0 1 1.03326 -1.9
3 496 2xau (A) 69 564 12.00 0 1 1.03538 0.53
3 215 2oxc (A) 62 266 32.00 0 1 0.922254 -1.33
5 217 3ber (A) 26 230 31.00 0 1 0.969254 -2.03
12 393 5ivl (B) 9 367 36.00 0 1 1.20993 -0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128567)

Species Accession Gene Product
Arabidopsis thaliana AT1G71370   DEAD-box ATP-dependent RNA helicase 49
Arabidopsis thaliana AT5G05450   DEAD-box ATP-dependent RNA helicase 18
Babesia bovis BBOV_III005220   DEAD/DEAH box domain containing protein
Candida albicans CaO19.13677   one of two almost identical potential DEAD box RNA helicase genes similar to S. cerevisiae SPB4 (YFL002C) ATP-dependent RNA heli
Candida albicans CaO19.11631   one of two almost identical potential DEAD box RNA helicase genes similar to S. cerevisiae SPB4 (YFL002C) ATP-dependent RNA heli
Candida albicans CaO19.6298   one of two almost identical potential DEAD box RNA helicase genes similar to S. cerevisiae SPB4 (YFL002C) ATP-dependent RNA heli
Candida albicans CaO19.4154   one of two almost identical potential DEAD box RNA helicase genes similar to S. cerevisiae SPB4 (YFL002C) ATP-dependent RNA heli
Caenorhabditis elegans CELE_ZK512.2   Protein ZK512.2, isoform B
Cryptosporidium parvum cgd3_1590   Spb4p, eIF4a-1-family RNA SFII helicase, DEXDc+HELICc domains
Dictyostelium discoideum DDB_G0291588   DEAD/DEAH box helicase
Drosophila melanogaster Dmel_CG9630   CG9630 gene product from transcript CG9630-RA
Echinococcus granulosus EgrG_000346000   ATP dependent RNA helicase DDX55
Entamoeba histolytica EHI_151190   DEAD/DEAH box helicase, putative
Echinococcus multilocularis EmuJ_000346000   ATP dependent RNA helicase DDX55
Giardia lamblia GL50803_15555   ATP-dependent RNA helicase
Homo sapiens ENSG00000111364   DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
Leishmania braziliensis LbrM.34.4010   ATP-dependent RNA helicase, putative
Leishmania donovani LdBPK_354080.1   ATP-dependent RNA helicase, putative
Leishmania infantum LinJ.35.4080   ATP-dependent RNA helicase, putative
Leishmania major LmjF.35.4030   ATP-dependent RNA helicase, putative
Leishmania mexicana LmxM.34.4030   ATP-dependent RNA helicase, putative
Mus musculus ENSMUSG00000029389   DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
Oryza sativa 4324413   Os01g0164500
Saccharomyces cerevisiae YFL002C   Spb4p
Schistosoma japonicum Sjp_0015780   ko:K01529 DEAD (Asp-Glu-Ala-Asp) box polypeptide 55 [EC:3.6.1.-], putative
Schistosoma japonicum Sjp_0202680   Centromere/kinetochore protein zw10 homolog, putative
Schistosoma mansoni Smp_125330   DEAD box ATP-dependent RNA helicase
Schmidtea mediterranea mk4.003740.02   ATP-dependent RNA helicase DDX55
Trypanosoma brucei gambiense Tbg972.9.6480   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma brucei Tb927.9.10960   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma cruzi TcCLB.508461.250   ATP-dependent RNA helicase, putative
Theileria parva TP02_0431   ATP-dependent RNA helicase, putative
Trichomonas vaginalis TVAG_172460   DEAD box ATP-dependent RNA helicase, putative

Essentiality

EHI_151190 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2300 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2300 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2300 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.2300 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_ZK512.2 Caenorhabditis elegans larval arrest wormbase
CELE_ZK512.2 Caenorhabditis elegans slow growth wormbase
YFL002C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_151190 (Entamoeba histolytica), DEAD/DEAH box helicase, putative
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