Detailed view for EHI_000410

Basic information

TDR Targets ID: 799637
Entamoeba histolytica, alcohol dehydrogenase, putative
Source Database / ID:  AmoebaDB  

pI: 6.7452 | Length (AA): 360 | MW (Da): 39476 | Paralog Number: 3

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00465   Iron-containing alcohol dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 355 3w5s (A) 1 344 15.00 0 1 1.17611 -0.65
6 356 1o2d (A) 2 357 30.00 0 1 1.3824 -0.83
6 356 3bfj (A) 6 387 20.00 0 1 1.2344 -0.55
66 174 4jdf (A) 216 310 34.00 0.47 0.6 0.440878 0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127140)

Species Accession Gene Product
Caenorhabditis elegans CELE_Y38F1A.6   Protein Y38F1A.6
Cryptosporidium hominis Chro.80199   aldehyde-alcohol dehydrogenase E
Cryptosporidium parvum cgd8_1720   acetaldehyde reductase plus alcohol dehydrogenase (AdhE) of possible bacterial origin
Dictyostelium discoideum DDB_G0290111   alcohol dehydrogenase iron-containing 1
Drosophila melanogaster Dmel_CG3425   Type III alcohol dehydrogenase
Escherichia coli b1241   fused acetaldehyde-CoA dehydrogenase/iron-dependent alcohol dehydrogenase/pyruvate-formate lyase deactivase
Escherichia coli b2453   ethanol dehydrogenase involved in ethanolamine utilization
Escherichia coli b3589   putative Fe-containing alcohol dehydrogenase, Pfam00465 family
Escherichia coli b2799   L-1,2-propanediol oxidoreductase
Entamoeba histolytica EHI_150490   aldehyde-alcohol dehydrogenase 2, putative
Entamoeba histolytica EHI_160940   aldehyde-alcohol dehydrogenase 2, putative
Entamoeba histolytica EHI_000410   alcohol dehydrogenase, putative
Entamoeba histolytica EHI_024240   aldehyde-alcohol dehydrogenase 2, putative
Giardia lamblia GL50803_13350   Alcohol dehydrogenase lateral transfer candidate
Giardia lamblia GL50803_3861   Alcohol dehydrogenase 3 lateral transfer candidate
Giardia lamblia GL50803_93358   Alcohol dehydrogenase
Homo sapiens ENSG00000147576   alcohol dehydrogenase, iron containing, 1
Leishmania braziliensis LbrM.30.2040   alcohol dehydrogenase, putative
Leishmania donovani LdBPK_302100.1   alcohol dehydrogenase, putative
Leishmania infantum LinJ.30.2100   alcohol dehydrogenase, putative
Leishmania major LmjF.30.2090   alcohol dehydrogenase, putative
Leishmania mexicana LmxM.29.2090   alcohol dehydrogenase, putative
Mus musculus ENSMUSG00000025911   alcohol dehydrogenase, iron containing, 1
Mycobacterium ulcerans MUL_3976   bifunctional acetaldehyde-CoA/alcohol dehydrogenase
Saccharomyces cerevisiae YGL256W   alcohol dehydrogenase ADH4
Schmidtea mediterranea mk4.001225.00   Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Trypanosoma cruzi TcCLB.506357.50   alcohol dehydrogenase, putative
Trypanosoma cruzi TcCLB.511277.60   alcohol dehydrogenase, putative
Trichomonas vaginalis TVAG_013020   alcohol acetaldehyde dehydrogenase, putative
Trichomonas vaginalis TVAG_327470   alcohol dehydrogenase, putative
Trichomonas vaginalis TVAG_422780   alcohol dehydrogenase, putative

Essentiality

EHI_000410 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1241 Escherichia coli non-essential goodall
b2453 Escherichia coli non-essential goodall
b2799 Escherichia coli non-essential goodall
b3589 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_000410 (Entamoeba histolytica), alcohol dehydrogenase, putative
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