Detailed view for EHI_108350

Basic information

TDR Targets ID: 799937
Entamoeba histolytica, hypothetical transmembrane protein
Source Database / ID:  AmoebaDB  

pI: 8.3202 | Length (AA): 258 | MW (Da): 29600 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 5

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF09335   SNARE associated Golgi protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
73 128 2prx (A) 43 109 35.00 0.79 0.07 0.281154 2.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127610)

Species Accession Gene Product
Arabidopsis thaliana AT1G22850   SNARE associated Golgi family protein
Arabidopsis thaliana AT5G19070   SNARE associated Golgi family protein
Arabidopsis thaliana AT1G03260   SNARE associated Golgi protein family
Babesia bovis BBOV_IV003160   conserved hypothetical protein
Candida albicans CaO19.5534   similar to S. cerevisiae TVP38 (YKR088C)
Candida albicans CaO19.12980   similar to S. cerevisiae YKR088C
Cryptosporidium hominis Chro.60544   multi-pass transmembrane protein
Cryptosporidium parvum cgd6_4730   hypothetical protein with 6 transmembrane domains
Dictyostelium discoideum DDB_G0292044   hypothetical protein
Dictyostelium discoideum DDB_G0268586   hypothetical protein
Dictyostelium discoideum DDB_G0275353   hypothetical protein
Dictyostelium discoideum DDB_G0291718   hypothetical protein
Drosophila melanogaster Dmel_CG11367   CG11367 gene product from transcript CG11367-RA
Escherichia coli b1750   TVP38/TMEM64 family inner membrane protein
Entamoeba histolytica EHI_167040   hypothetical protein, conserved
Entamoeba histolytica EHI_108350   hypothetical transmembrane protein
Entamoeba histolytica EHI_049010   hypothetical protein
Homo sapiens ENSG00000180694   transmembrane protein 64
Mus musculus ENSMUSG00000043252   transmembrane protein 64
Mycobacterium tuberculosis Rv1491c   Conserved membrane protein
Mycobacterium ulcerans MUL_1504   hypothetical protein
Neospora caninum NCLIV_012810   hypothetical protein
Oryza sativa 4324879   Os01g0884900
Oryza sativa 4344144   Os07g0655900
Oryza sativa 4344014   Os07g0634000
Plasmodium berghei PBANKA_1141500   SNARE associated Golgi protein, putative
Plasmodium falciparum PF3D7_1365700   SNARE associated Golgi protein, putative
Plasmodium knowlesi PKNH_1105600   SNARE associated Golgi protein, putative
Plasmodium vivax PVX_115245   SNARE associated Golgi protein, putative
Plasmodium yoelii PY02843   hypothetical protein
Saccharomyces cerevisiae YKR088C   Tvp38p
Toxoplasma gondii TGME49_301350   SNARE associated protein
Theileria parva TP01_1067   hypothetical protein, conserved

Essentiality

EHI_108350 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1750 Escherichia coli non-essential goodall
PBANKA_1141500 Plasmodium berghei Essential plasmo
TGME49_301350 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_108350 (Entamoeba histolytica), hypothetical transmembrane protein
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