Detailed view for EHI_069480

Basic information

TDR Targets ID: 800591
Entamoeba histolytica, HEAT repeat domain containing protein
Source Database / ID:  AmoebaDB  

pI: 8.6206 | Length (AA): 707 | MW (Da): 78375 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
11 276 2rku (A) 37 311 13.00 0 0.83 0.501238 -0.67
17 551 3vwa (A) 11 544 19.00 0 1 0.919719 -0.08
124 199 4y83 (A) 230 286 40.00 0.33 0.21 -0.0102035 1.11
545 685 2jks (A) 6 165 12.00 0 0.1 0.378234 -1.3
565 705 4qv2 (A) 272 447 14.00 0 0.02 0.344234 -0.76
652 699 2f3j (A) 29 68 53.00 0.75 0.22 0.352192 0

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128179)

Species Accession Gene Product
Arabidopsis thaliana AT2G40730   cytoplasmic tRNA export protein
Babesia bovis BBOV_IV008520   protein kinase, putative
Brugia malayi Bm1_15080   105-kDa kinase-like protein
Candida albicans CaO19.3744   similar to suppressor of GTPase mutant
Candida albicans CaO19.11229   similar to suppressor of GTPase mutant
Caenorhabditis elegans CELE_W07G4.3   Protein W07G4.3
Cryptosporidium hominis Chro.80285   protein kinase
Cryptosporidium parvum cgd8_2440   Ser/Thr protein kinase
Dictyostelium discoideum DDB_G0267540   N-terminal kinase-like protein
Drosophila melanogaster Dmel_CG1973   CG1973 gene product from transcript CG1973-RB
Echinococcus granulosus EgrG_000530320   N terminal kinase protein like
Entamoeba histolytica EHI_069480   HEAT repeat domain containing protein
Entamoeba histolytica EHI_146520   HEAT repeat domain containing protein
Echinococcus multilocularis EmuJ_000530320   scy1(yeast) protein kinase-like
Homo sapiens ENSG00000142186   SCY1-like 1 (S. cerevisiae)
Leishmania braziliensis LbrM.21.1060   hypothetical protein, conserved
Leishmania donovani LdBPK_211050.1   hypothetical protein, conserved
Leishmania infantum LinJ.21.1050   hypothetical protein, conserved
Leishmania major LmjF.21.0873   hypothetical protein, conserved
Leishmania mexicana LmxM.21.0873   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00862   hypothetical protein
Loa Loa (eye worm) LOAG_15587   hypothetical protein
Loa Loa (eye worm) LOAG_06853   SCY1 protein kinase
Mus musculus ENSMUSG00000024941   SCY1-like 1 (S. cerevisiae)
Neospora caninum NCLIV_010260   hypothetical protein
Oryza sativa 4327606   Os01g0819900
Plasmodium berghei PBANKA_1103200   protein kinase, putative
Plasmodium falciparum PF3D7_0503500   protein kinase, putative
Plasmodium knowlesi PKNH_1030100   protein kinase, putative
Plasmodium vivax PVX_097750   protein kinase, putative
Plasmodium yoelii PY04701   Homo sapiens HT019
Saccharomyces cerevisiae YOR112W   Cex1p
Schistosoma japonicum Sjp_0045270   ko:K08876 SCY1-like, putative
Schistosoma mansoni Smp_176440   protein kinase
Schmidtea mediterranea mk4.002607.00   Protein kinase
Schmidtea mediterranea mk4.002607.02  
Schmidtea mediterranea mk4.002607.01   Protein kinase
Trypanosoma brucei gambiense Tbg972.10.1520   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.1320   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_10_1110   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509693.160   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506457.120   hypothetical protein, conserved
Toxoplasma gondii TGME49_320000   SCY kinase (incomplete catalytic triad)
Theileria parva TP01_0925   protein kinase, putative
Trichomonas vaginalis TVAG_469010   conserved hypothetical protein

Essentiality

EHI_069480 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1320 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.1320 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.1320 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.1320 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1103200 Plasmodium berghei Dispensable plasmo
TGME49_320000 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus SCY1-like 1 (S. cerevisiae) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0088 0.4477 0.5
0.0016 0.5 0.5
0.0023 0.5 0.5
0.0037 1 0.5
0.0007 0.5 0.5
0.0042 0.5 0.5
0.0069 0.3067 0.5
0.0029 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0
0.0018 0.5 0.5
0.0004 0.5 0.5
0.0092 1 0.5
0.0003 0.5 0.5
0.0011 1 0.5
0.0033 0.5 0.5
0.0012 0.5 0.5
0.0081 0.5 0.5
0.0062 0.6935 0
0.0007 0.5 0.5
0.0061 0.6883 0.6247
0.0059 1 1
0.0008 0.5 0.5
0.0007 0.5 0.5
0.0067 0.5 0.5
0.0036 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.3167
0.0039 0.9485 0.5
0.0059 1 1
0.0039 0.5 0.5
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0093 0.8828 0
0.0063 1 1
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0091 1 0.5
0.0022 0.5 0.5
0.0064 0.3377 0.8757
0.0081 1 0.5
0.0033 1 1
0.0059 1 1
0.0056 1 0.5
0.0063 0.7244 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_069480 (Entamoeba histolytica), HEAT repeat domain containing protein
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