Detailed view for EHI_051060

Basic information

TDR Targets ID: 800653
Entamoeba histolytica, pyruvate:ferredoxin oxidoreductase
Source Database / ID:  AmoebaDB  

pI: 6.7701 | Length (AA): 1162 | MW (Da): 127636 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01558   Pyruvate ferredoxin/flavodoxin oxidoreductase
PF01855   Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg
PF02775   Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
PF10371   Domain of unknown function
PF12838   4Fe-4S dicluster domain
PF17147   Pyruvate:ferredoxin oxidoreductase core domain II

Gene Ontology

Mouse over links to read term descriptions.
GO:0016903   oxidoreductase activity, acting on the aldehyde or oxo group of donors  
GO:0051536   iron-sulfur cluster binding  
GO:0030976   thiamin pyrophosphate binding  
GO:0016491   oxidoreductase activity  
GO:0005506   iron ion binding  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0022900   electron transport chain  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 1160 2c42 (A) 5 1168 51.00 0 1 1.64448 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129721)

Species Accession Gene Product
Cryptosporidium parvum cgd4_690   pyruvate:ferredoxin oxidoreductase/NADPH-cytochrome P450 reductase PNO
Escherichia coli b1378   pyruvate-flavodoxin oxidoreductase
Entamoeba histolytica EHI_051060   pyruvate:ferredoxin oxidoreductase
Giardia lamblia GL50803_114609   Pyruvate-flavodoxin oxidoreductase
Giardia lamblia GL50803_17063   Pyruvate-flavodoxin oxidoreductase
Treponema pallidum TP0939   pyruvate oxidoreductase
Trichomonas vaginalis TVAG_198110   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_466790   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_230580   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_105770   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_254890   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_096520   pyruvate-flavodoxin oxidoreductase, putative
Trichomonas vaginalis TVAG_242960   pyruvate-flavodoxin oxidoreductase, putative

Essentiality

EHI_051060 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1378 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_051060 (Entamoeba histolytica), pyruvate:ferredoxin oxidoreductase
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