Detailed view for EHI_000490

Basic information

TDR Targets ID: 802273
Entamoeba histolytica, PRP38 family protein
Source Database / ID:  AmoebaDB  

pI: 7.7267 | Length (AA): 190 | MW (Da): 22813 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03371   PRP38 family

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Spliceosome (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 166 4rz9 (A) 15 179 44.00 0 1 1.56012 -1.61
3 188 5f5t (A) 30 218 34.00 0 1 1.47515 -0.82
122 154 5dzu (A) 123 151 45.00 0.58 0.07 0.173384 3.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128828)

Species Accession Gene Product
Arabidopsis thaliana AT2G40650   pre-mRNA-splicing factor 38A
Babesia bovis BBOV_IV007490   pre-mRNA splicing factor, putative
Brugia malayi Bm1_26600   GM13395p
Candida albicans CaO19.9839   PRP38 family protein similar to S. cerevisiae PRP38 (YGR075C) RNA splicing factor associated with the U4/U6.U5 tri-snRNP
Candida albicans CaO19.2303   PRP38 family protein similar to S. cerevisiae PRP38 (YGR075C) RNA splicing factor associated with the U4/U6.U5 tri-snRNP
Caenorhabditis elegans CELE_D1054.14   Protein PRP-38
Cryptosporidium hominis Chro.30226   hypothetical protein
Cryptosporidium parvum cgd3_1920   possible PRP38 pre-mRNA splicing factor family member
Dictyostelium discoideum DDB_G0274361   pre-mRNA processing factor 38
Drosophila melanogaster Dmel_CG30342   pre-mRNA processing factor 38
Echinococcus granulosus EgrG_000425700   pre mRNA splicing factor 38A
Entamoeba histolytica EHI_000490   PRP38 family protein
Echinococcus multilocularis EmuJ_000425700   pre mRNA splicing factor 38A
Giardia lamblia GL50803_9259   Hypothetical protein
Homo sapiens 84950   pre-mRNA processing factor 38A
Loa Loa (eye worm) LOAG_11806   pre-mRNA-splicing factor 38A
Loa Loa (eye worm) LOAG_00678   hypothetical protein
Mus musculus ENSMUSG00000063800   PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Neospora caninum NCLIV_048000   PRP38 family domain-containing protein, putative
Oryza sativa 4346374   Os09g0103600
Plasmodium berghei PBANKA_0915800   pre-mRNA-splicing factor 38A, putative
Plasmodium falciparum PF3D7_1132600   pre-mRNA-splicing factor 38A, putative
Plasmodium knowlesi PKNH_0930800   pre-mRNA-splicing factor 38A, putative
Plasmodium vivax PVX_092235   pre-mRNA-splicing factor 38A, putative
Plasmodium yoelii PY05953   hypothetical protein
Schistosoma japonicum Sjp_0079920   Pre-mRNA-splicing factor 38A, putative
Schistosoma mansoni Smp_046980   hypothetical protein
Toxoplasma gondii TGME49_266030   pre-mRNA splicing factor, putative
Theileria parva TP03_0267   hypothetical protein, conserved
Trichomonas vaginalis TVAG_408720   spbc19c2.08 protein, putative

Essentiality

EHI_000490 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_D1054.14 Caenorhabditis elegans embryonic lethal wormbase
CELE_D1054.14 Caenorhabditis elegans larval arrest wormbase
CELE_D1054.14 Caenorhabditis elegans slow growth wormbase
CELE_D1054.14 Caenorhabditis elegans sterile wormbase
TGME49_266030 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_000490 (Entamoeba histolytica), PRP38 family protein
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