Detailed view for EHI_155360

Basic information

TDR Targets ID: 803625
Entamoeba histolytica, protein kinase, putative
Source Database / ID:  AmoebaDB  

pI: 9.0098 | Length (AA): 231 | MW (Da): 26650 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 226 3enh (A) 314 531 35.00 0 1 1.2522 0.02
23 227 4ww7 (A) 22 257 39.00 0 1 1.18715 -0.42
44 166 3cok (A) 19 158 28.00 0.31 0.6 0.668168 0.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127673)

Species Accession Gene Product
Arabidopsis thaliana AT5G26110   protein kinase family protein
Babesia bovis BBOV_III004260   conserved hypothetical protein
Brugia malayi Bm1_51105   Nori-2 protein
Candida albicans CaO19.4252   similar to apple calcium/calmodulin-binding protein kinase
Candida albicans CaO19.11727   similar to apple calcium/calmodulin-binding protein kinase
Caenorhabditis elegans CELE_F52C12.6   Protein F52C12.6
Cryptosporidium parvum cgd8_4700   hypothetical protein
Dictyostelium discoideum DDB_G0279977   BUD32 family protein kinase
Drosophila melanogaster Dmel_CG10673   p53-related protein kinase ortholog
Echinococcus granulosus EgrG_001096800   testis expressed sequence 9 protein
Echinococcus granulosus EgrG_001096900   tp53 regulating kinase
Entamoeba histolytica EHI_155360   protein kinase, putative
Echinococcus multilocularis EmuJ_001096900   tp53 regulating kinase
Echinococcus multilocularis EmuJ_001096800   testis expressed sequence 9 protein
Giardia lamblia GL50803_16796   TP53 regulating kinase
Homo sapiens ENSG00000172315   TP53 regulating kinase
Leishmania braziliensis LbrM.25.0750   protein kinase, putative
Leishmania donovani LdBPK_250900.1   protein kinase, putative
Leishmania infantum LinJ.25.0900   protein kinase, putative
Leishmania major LmjF.25.0870   protein kinase, putative
Leishmania mexicana LmxM.25.0870   protein kinase, putative
Loa Loa (eye worm) LOAG_09194   BUD32 protein kinase
Mus musculus ENSMUSG00000039725   RIKEN cDNA 2810408M09 gene
Mus musculus ENSMUSG00000042854   transformation related protein 53 regulating kinase
Neospora caninum NCLIV_067110   hypothetical protein
Oryza sativa 4348657   Os10g0422300
Plasmodium berghei PBANKA_0805600   EKC/KEOPS complex subunit BUD32, putative
Plasmodium falciparum PF3D7_0708300   EKC/KEOPS complex subunit BUD32
Plasmodium knowlesi PKNH_0106900   EKC/KEOPS complex subunit BUD32, putative
Plasmodium vivax PVX_087945   EKC/KEOPS complex subunit BUD32, putative
Plasmodium yoelii PY00132   Unknown protein
Saccharomyces cerevisiae YGR262C   Bud32p
Schistosoma japonicum Sjp_0027460   ko:K08851 TP53 regulating kinase, putative
Schistosoma mansoni Smp_139630   protein kinase
Schmidtea mediterranea mk4.005629.05  
Trypanosoma brucei gambiense Tbg972.11.890   protein kinase, putative
Trypanosoma brucei Tb927.11.850   Atypical serine/threonine protein kinase BUD32, putative
Trypanosoma congolense TcIL3000_0_07170   protein kinase, putative
Trypanosoma cruzi TcCLB.506925.260   Atypical serine/threonine protein kinase BUD32, putative
Toxoplasma gondii TGME49_278900   protein kinase, putative
Theileria parva TP02_0241   hypothetical protein, conserved
Trichomonas vaginalis TVAG_443660   TKL family protein kinase
Trichomonas vaginalis TVAG_344730   TKL family protein kinase

Essentiality

EHI_155360 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0290 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0290 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0290 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0290 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0805600 Plasmodium berghei Essential plasmo
TGME49_278900 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0064 0.3377 0.8757
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0
0.0093 0.8828 0
0.0032 0.5 0.5
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0061 0.6883 0.6247
0.0026 0.5 0.5
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0059 1 1
0.0062 0.6935 0
0.0092 1 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.3167
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0007 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0059 1 1
0.0069 0.3067 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_155360 (Entamoeba histolytica), protein kinase, putative
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