Detailed view for EHI_137090

Basic information

TDR Targets ID: 804760
Entamoeba histolytica, TFIID subunit, putative
Source Database / ID:  AmoebaDB  

pI: 5.01 | Length (AA): 174 | MW (Da): 19726 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04658   TAFII55 protein conserved region

Gene Ontology

Mouse over links to read term descriptions.
GO:0005669   transcription factor TFIID complex  
GO:0006367   transcription initiation from RNA polymerase II promoter  

Metabolic Pathways

Basal transcription factors (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
30 159 4rgw (B) 12 154 27.00 0 1 0.938226 0.26
30 159 4rgw (B) 12 154 30.00 0.0000064 0.97 0.942026 0.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_128786)

Species Accession Gene Product
Arabidopsis thaliana AT1G55300   TBP-associated factor 7
Brugia malayi Bm1_55960   TAFII55 protein conserved region containing protein
Candida albicans CaO19.9147   component of the TAF(II) complex
Candida albicans CaO19.1574   component of the TAF(II) complex
Caenorhabditis elegans CELE_Y111B2A.16   Protein TAF-7.2
Caenorhabditis elegans CELE_F54F7.1   Protein TAF-7.1
Dictyostelium discoideum DDB_G0271338   TFIID subunit
Drosophila melanogaster Dmel_CG2670   TBP-associated factor 7
Echinococcus granulosus EgrG_000514850   transcription initiation factor tfiid 55 kD subunit-related
Entamoeba histolytica EHI_137090   TFIID subunit, putative
Echinococcus multilocularis EmuJ_000514850   transcription initiation factor tfiid 55 kD subunit-related
Homo sapiens ENSG00000178913   TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
Homo sapiens ENSG00000102387   TAF7-like RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50kDa
Loa Loa (eye worm) LOAG_03972   hypothetical protein
Mus musculus ENSMUSG00000074734   RIKEN cDNA 4933416C03 gene
Mus musculus ENSMUSG00000051316   TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor
Oryza sativa 4338489   Os05g0347000
Onchocerca volvulus OVOC8551   Transcription initiation factor TFIID subunit 7 homolog
Saccharomyces cerevisiae YMR227C   Taf7p
Schistosoma japonicum Sjp_0071650   ko:K03132 transcription initiation factor TFIID subunit D6, putative
Schistosoma mansoni Smp_169300   transcription initiation factor tfiid 55 kD subunit-related
Schmidtea mediterranea mk4.001917.05  
Schmidtea mediterranea mk4.004390.02  
Trichomonas vaginalis TVAG_040830   transcription initiation factor TFIID subunit, putative

Essentiality

EHI_137090 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YMR227C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_137090 (Entamoeba histolytica), TFIID subunit, putative
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