Detailed view for EHI_127410

Basic information

TDR Targets ID: 805158
Entamoeba histolytica, protein kinase domain containing protein
Source Database / ID:  AmoebaDB  

pI: 6.6865 | Length (AA): 519 | MW (Da): 58755 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 302 4w9w (A) 39 340 39.00 0 1 1.11619 -1.02
3 315 5te0 (A) 36 345 37.00 0 1 1.08718 -1.03
8 414 5t6a (A) 46 476 19.00 0 1 0.9223 0.03
118 229 3dls (A) 1108 1205 33.00 0.0094 0.54 0.3021 0.6

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127425)

Species Accession Gene Product
Arabidopsis thaliana AT2G32850   protein kinase superfamily protein
Brugia malayi Bm1_37065   Protein kinase domain containing protein
Candida albicans CaO19.5357   Ark-family kinase-like protein
Candida albicans CaO19.2605   possible serine/threonine kinase
Candida albicans CaO19.10136   possible serine/threonine kinase
Candida albicans CaO19.12817   Ark-family kinase-like protein
Caenorhabditis elegans CELE_F35G12.3   Protein SEL-5, isoform B
Dictyostelium discoideum DDB_G0276461   NAK family protein kinase
Dictyostelium discoideum DDB_G0280111   NAK family protein kinase
Drosophila melanogaster Dmel_CG10637   Numb-associated kinase
Echinococcus granulosus EgrG_000165700   AP2 associated protein kinase 1
Entamoeba histolytica EHI_103290   protein kinase, putative
Entamoeba histolytica EHI_127410   protein kinase domain containing protein
Echinococcus multilocularis EmuJ_000165700   AP2 associated protein kinase 1
Giardia lamblia GL50803_12223   Kinase, NAK
Homo sapiens ENSG00000138756   BMP2 inducible kinase
Homo sapiens ENSG00000115977   AP2 associated kinase 1
Leishmania braziliensis LbrM.20.0110   protein kinase, putative
Leishmania donovani LdBPK_340030.1   protein kinase, putative
Leishmania infantum LinJ.34.0030   protein kinase, putative
Leishmania major LmjF.34.0030   protein kinase, putative
Leishmania mexicana LmxM.33.0030   protein kinase, putative
Loa Loa (eye worm) LOAG_05227   NAK/BIKE protein kinase
Mus musculus ensembl-mmu:ENSMUSG00000034663   BMP2 inducible kinase
Mus musculus 102637099   uncharacterized LOC102637099
Mus musculus ENSMUSG00000057230   AP2 associated kinase 1
Neospora caninum NCLIV_050490   hypothetical protein
Oryza sativa 4346601   Os09g0279100
Plasmodium berghei PBANKA_1460500   serine/threonine protein kinase, putative
Plasmodium falciparum PF3D7_1247500   serine/threonine protein kinase, putative
Plasmodium knowlesi PKNH_1467200   serine/threonine protein kinase, putative
Plasmodium vivax PVX_101265   cyclin g-associated kinase, putative
Plasmodium yoelii PY06849   hypothetical protein
Saccharomyces cerevisiae YBR059C   Akl1p
Saccharomyces cerevisiae YIL095W   Prk1p
Saccharomyces cerevisiae YNL020C   Ark1p
Schistosoma japonicum Sjp_0086670   ko:K08854 BMP2 inducible kinase, putative
Schistosoma japonicum Sjp_0107280   AP2-associated protein kinase 1, putative
Schistosoma mansoni Smp_176750   serine/threonine protein kinase
Schmidtea mediterranea mk4.015925.00  
Schmidtea mediterranea mk4.000160.18  
Schmidtea mediterranea mk4.006232.02  
Schmidtea mediterranea mk4.006232.01  
Trypanosoma cruzi TcCLB.506953.30   serine/threonine-protein kinase NAK, putative
Trypanosoma cruzi TcCLB.509287.20   serine/threonine-protein kinase NAK, putative
Toxoplasma gondii TGME49_236240   Tyrosine kinase-like (TKL) protein
Trichomonas vaginalis TVAG_144060   CAMK family protein kinase
Trichomonas vaginalis TVAG_475520   CAMK family protein kinase

Essentiality

EHI_127410 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_F35G12.3 Caenorhabditis elegans embryonic lethal wormbase
YNL020C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1460500 Plasmodium berghei Slow plasmo
TGME49_236240 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens AP2 associated kinase 1 Compounds References
Homo sapiens BMP2 inducible kinase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 25.9% 170 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0056 1 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0
0.0064 0.3377 0.8757
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0007 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0059 1 1
0.0069 0.3067 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0004 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0092 1 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.3167
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0059 1 1
0.0062 0.6935 0
0.0066 0.3101 0
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.6247
0.0026 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier EHI_127410 (Entamoeba histolytica), protein kinase domain containing protein
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