Detailed view for EHI_003930

Basic information

TDR Targets ID: 805828
Entamoeba histolytica, I/LWEQ domain protein
Source Database / ID:  AmoebaDB  

pI: 5.5062 | Length (AA): 995 | MW (Da): 114979 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01608   I/LWEQ domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0030276   clathrin binding  
GO:0003779   actin binding  
GO:0006897   endocytosis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
313 995 1tr2 (A) 226 895 13.00 0 1 0.602332 0.34
348 584 4tql (A) 11 238 23.00 0 0.97 0.492091 -1.64
391 574 4uos (A) 3 184 21.00 0 0.79 0.496825 -2.44
762 941 2jsw (A) 2305 2482 39.00 0 1 0.744905 -1.28

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite, Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129034)

Species Accession Gene Product
Brugia malayi Bm1_18970   ANTH domain containing protein
Candida albicans CaO19_7201   hypothetical protein
Candida albicans CaO19.7201   cytoskeletal structural protein
Caenorhabditis elegans CELE_ZK370.3   Protein HIPR-1, isoform B
Dictyostelium discoideum DDB_G0285703   ENTH domain-containing protein
Drosophila melanogaster Dmel_CG10971   Huntingtin interacting protein 1
Echinococcus granulosus EgrG_000728100   huntingtin interacting protein 1
Entamoeba histolytica EHI_003930   I/LWEQ domain protein
Echinococcus multilocularis EmuJ_000728100   huntingtin interacting protein 1
Homo sapiens ENSG00000127946   huntingtin interacting protein 1
Homo sapiens ENSG00000130787   huntingtin interacting protein 1 related
Loa Loa (eye worm) LOAG_06138   hypothetical protein
Mus musculus ENSMUSG00000000915   huntingtin interacting protein 1 related
Mus musculus ENSMUSG00000039959   huntingtin interacting protein 1
Onchocerca volvulus OVOC8000   Huntingtin interacting protein 1 homolog
Saccharomyces cerevisiae YNL243W   Sla2p
Schistosoma japonicum Sjp_0041470   Huntingtin-interacting protein 1, putative
Schistosoma japonicum Sjp_0313870   ko:K04559 huntingtin interacting protein 1, putative
Schistosoma mansoni Smp_181350   huntingtin interacting protein-related
Schmidtea mediterranea mk4.000805.08   Huntingtin interacting protein 1
Schmidtea mediterranea mk4.000805.10   Huntington inteteracting protein-related

Essentiality

EHI_003930 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YNL243W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_003930 (Entamoeba histolytica), I/LWEQ domain protein
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