Detailed view for GL50803_11449

Basic information

TDR Targets ID: 811763
Giardia lamblia, ATPase of the PP-loop superfamily implicated in cell cycle control, putative
Source Database / ID:  GiardiaDB  

pI: 8.727 | Length (AA): 351 | MW (Da): 39004 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01171   PP-loop family

Gene Ontology

Mouse over links to read term descriptions.
GO:0034227   tRNA thio-modification  
GO:0000049   tRNA binding  
GO:0008033   tRNA processing  
GO:0002098   tRNA wobble uridine modification  

Metabolic Pathways

Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 338 3vrh (A) 1 293 29.00 0 1 1.12876 0.08
3 350 5b4f (A) 3 302 33.00 0 1 1.12485 0.39
3 351 5b4f (A) 3 313 27.00 0.000000000039 1 1.0673 0.33
32 117 3q4g (A) 18 108 24.00 0 0.59 0.511814 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127415)

Species Accession Gene Product
Arabidopsis thaliana AT2G44270   ROL5, repressor of LRX1
Babesia bovis BBOV_III004480   conserved hypothetical protein
Babesia bovis BBOV_III004460   PP-loop family protein
Babesia bovis BBOV_III004500   conserved hypothetical protein
Brugia malayi Bm1_38855   n-type ATP pyrophosphatase-like
Candida albicans CaO19.4634   similar to S. cerevisiae YGL211W
Candida albicans CaO19.12104   similar to S. cerevisiae YGL211W
Caenorhabditis elegans CELE_F29C4.6   Protein TUT-1
Cryptosporidium hominis Chro.50341   cancer-associated gene protein like (41.3 kD) (4A872)
Cryptosporidium parvum cgd5_520   MJ1157-like thiouridine synthase (Pploop atpase) plus Zn ribbon. involved in RNA metabolism.
Dictyostelium discoideum DDB_G0282921   ATP-binding domain-containing protein 3
Drosophila melanogaster Dmel_CG8078   CG8078 gene product from transcript CG8078-RA
Echinococcus granulosus EgrG_000963000   cytoplasmic tRNA 2 thiolation protein 1
Entamoeba histolytica EHI_108760   PP-loop family protein
Echinococcus multilocularis EmuJ_000963000   cytoplasmic tRNA 2 thiolation protein 1
Giardia lamblia GL50803_11449   ATPase of the PP-loop superfamily implicated in cell cycle control, putative
Homo sapiens ENSG00000142544   cytosolic thiouridylase subunit 1
Leishmania braziliensis LbrM.35.6430   hypothetical protein, conserved
Leishmania donovani LdBPK_366380.1   TIGR00269 family protein, putative
Leishmania major LmjF.36.6120   hypothetical protein, conserved
Leishmania mexicana LmxM.36.6120   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00156   tRNA 2-thiolation protein 1
Mus musculus ENSMUSG00000038888   cytosolic thiouridylase subunit 1 homolog (S. pombe)
Neospora caninum NCLIV_053470   PP-loop domain protein, related
Oryza sativa 4330815   Os02g0762300
Oryza sativa 9269998   Os01g0598900
Plasmodium berghei PBANKA_0110900   cytoplasmic tRNA 2-thiolation protein 1, putative
Plasmodium falciparum PF3D7_0612600   cytoplasmic tRNA 2-thiolation protein 1, putative
Plasmodium knowlesi PKNH_1137400   cytoplasmic tRNA 2-thiolation protein 1, putative
Plasmodium vivax PVX_113770   hypothetical protein, conserved
Plasmodium yoelii PY03101   Uncharacterized protein family UPF0021
Saccharomyces cerevisiae YGL211W   Ncs6p
Schistosoma japonicum Sjp_0035810   Protein NCS6, putative
Schistosoma mansoni Smp_040560   cancer-associatedprotein protein
Schmidtea mediterranea mk4.004114.01   Cytoplasmic tRNA 2-thiolation protein 1
Schmidtea mediterranea mk4.004114.00   Cytoplasmic tRNA 2-thiolation protein 1
Schmidtea mediterranea mk4.004114.02  
Trypanosoma brucei gambiense Tbg972.10.10540   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.8690   TIGR00269 family protein, putative
Trypanosoma congolense TcIL3000_10_7480   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508727.30   TIGR00269 family protein, putative
Trypanosoma cruzi TcCLB.503797.10   TIGR00269 family protein, putative
Toxoplasma gondii TGME49_309020   PP-loop family protein
Theileria parva TP02_0798   hypothetical protein, conserved
Trichomonas vaginalis TVAG_494120   cell cycle protein mesj, putative

Essentiality

GL50803_11449 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.8690 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8690 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8690 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.8690 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_309020 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier GL50803_11449 (Giardia lamblia), ATPase of the PP-loop superfamily implicated in cell cycle control, putative
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