Detailed view for LbrM.20.4950

Basic information

TDR Targets ID: 815612
Leishmania braziliensis, protein kinase, putative
Source Database / ID: 

pI: 6.4712 | Length (AA): 3938 | MW (Da): 415931 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00027   Cyclic nucleotide-binding domain
PF00069   Protein kinase domain
PF00989   PAS fold

Gene Ontology

Mouse over links to read term descriptions.
GO:0006355   regulation of transcription, DNA-dependent  
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 17 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
55 208 4hji (A) 14 161 7.00 0 0 -0.0133939 -0.27
1164 1361 2mu3 (A) 4 198 32.00 0.26 0.05 0.215879 0.65
1967 2128 2ptm (A) 492 656 16.00 0.0000013 0.21 0.101938 0.4
1973 2259 2qcs (B) 92 345 19.00 0 1 0.10268 0.9
1995 2240 5kbf (A) 164 417 20.00 0 1 0.273268 -0.21
2013 2112 2pqq (A) 3 104 29.00 0.000000083 0.98 0.319194 -0.06
2124 2240 5jix (A) 269 401 20.00 0 0.48 0.213511 -0.86
2126 2198 3dkw (A) 1 73 30.00 0.0003 0.23 0.234337 0.87
3421 3809 2fo0 (A) 154 528 21.00 0 1 0.219581 0.59
3489 3884 2rsv (A) 0 394 19.00 0.0000000000064 0.7 0.0633587 1.27
3509 3810 3fxz (A) 249 538 31.00 0 1 0.473489 -0.47
3520 3730 2oid (A) 174 385 39.00 0 0.32 0.0401805 1.01
3520 3931 3hx4 (A) 41 487 23.00 0 1 0.221422 0.33
3525 3827 3ggf (A) 19 300 34.00 0 1 0.347743 0.29
3534 3808 3a7i (A) 28 290 37.00 0 1 0.478432 -0.23
3625 3817 2pml (X) 143 341 29.00 0.0000059 0.97 0.35261 -0.31
3848 3931 1dtl (A) 49 143 18.00 0.83 0.04 0.180131 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_145823)

Species Accession Gene Product
Leishmania braziliensis LbrM.20.4950   protein kinase, putative
Leishmania donovani LdBPK_200780.1   protein kinase, putative
Leishmania infantum LinJ.20.0780   protein kinase, putative
Leishmania major LmjF.20.0770   protein kinase, putative
Leishmania mexicana LmxM.20.0770   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.1.760   protein kinase, putative
Trypanosoma brucei Tb11.v5.0534   protein kinase, putative
Trypanosoma brucei Tb927.1.1530   STE group serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_0_00130   protein kinase, putative
Trypanosoma congolense TcIL3000_0_12910   protein kinase, putative
Trypanosoma cruzi TcCLB.506477.60   STE group serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.508995.10   STE group serine/threonine-protein kinase, putative

Essentiality

LbrM.20.4950 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.1530 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.1530 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.1530 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1530 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 29.7% 276 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 21.8% 275 aa Compounds References
Patiria pectinifera Cdc2 300 aa 27.9% 247 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 29.7% 286 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 26.4% 201 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 26.4% 296 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LbrM.20.4950 (Leishmania braziliensis), protein kinase, putative
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