Detailed view for PF3D7_0705400

Basic information

TDR Targets ID: 823
Plasmodium falciparum, DNA replication licensing factor MCM7
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.2695 | Length (AA): 821 | MW (Da): 94237 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00493   MCM2/3/5 family
PF14551   MCM N-terminal domain
PF17207   MCM OB domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0042555   MCM complex  
GO:0005634   nucleus  
GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0003678   DNA helicase activity  
GO:0003677   DNA binding  
GO:0000166   nucleotide binding  
GO:0006270   DNA replication initiation  
GO:0006260   DNA replication  

Metabolic Pathways

DNA replication (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
118 353 1ltl (A) 11 236 19.00 0 0.94 0.32 0.71
397 726 1g8p (A) 18 347 17.00 0 1 0.23 0.32
111 355 1ltl (A) 4 238 21.00 0.00000053 0.84 0.468517 0.57
113 722 4r7y (A) 7 1965 30.00 0 1 0.998096 0.78
187 722 4r7y (A) 87 1965 38.00 0 1 0.931962 0.83
187 712 4fdg (B) 87 592 40.00 0 1 0.894782 1.38
660 744 2r44 (A) 243 329 15.00 0.72 0.06 0.275132 -0.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, sporozoite, early schizont, late schizont, late trophozoite, Oocyst, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, merozoite, early trophozoite, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile late ring, Ring. PlasmoDB Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_128241)

Species Accession Gene Product
Arabidopsis thaliana AT4G02060   protein PROLIFERA
Babesia bovis BBOV_IV007280   ATP dependent DNA helicase, putative
Brugia malayi Bm1_04660   replication licensing factor MCM7
Brugia malayi Bm1_31460   replication licensing factor MCM7
Candida albicans CaO19.7832   chromatin binding/ DNA replication
Candida albicans CaO19.202   chromatin binding/ DNA replication
Candida albicans CaO19.7831   one of three C. albicans genes with potential N terminal DUF202 similar to yidH (E. coli )
Candida albicans CaO19.201   one of three C. albicans genes with potential N terminal DUF202 similar to yidH (E. coli )
Caenorhabditis elegans CELE_F32D1.10   Protein MCM-7
Cryptosporidium hominis Chro.40117   minichromosome maintenance protein mcm7p
Cryptosporidium parvum cgd4_970   DNA replication licensing factor MCM7 like AAA+ ATpase
Dictyostelium discoideum DDB_G0282933   MCM family protein
Drosophila melanogaster Dmel_CG4978   Minichromosome maintenance 7
Echinococcus granulosus EgrG_000510700   DNA replication licensing factor mcm7
Entamoeba histolytica EHI_158110   DNA replication licensing factor, putative
Echinococcus multilocularis EmuJ_000510700   DNA replication licensing factor mcm7
Giardia lamblia GL50803_10765   MCM7
Homo sapiens ENSG00000166508   minichromosome maintenance complex component 7
Leishmania braziliensis LbrM.32.3220   minichromosome maintenance (MCM) complex subunit, putative
Leishmania donovani LdBPK_323120.1   minichromosome maintenance (MCM) complex subunit, putative
Leishmania infantum LinJ.32.3120   minichromosome maintenance (MCM) complex subunit, putative
Leishmania major LmjF.32.2960   minichromosome maintenance (MCM) complex subunit, putative
Leishmania mexicana LmxM.31.2960   minichromosome maintenance (MCM) complex subunit, putative
Loa Loa (eye worm) LOAG_04710   replication licensing factor MCM7
Mus musculus ENSMUSG00000029730   minichromosome maintenance deficient 7 (S. cerevisiae)
Neospora caninum NCLIV_051090   DNA replication licensing factor, putative
Oryza sativa 4352514   Os12g0560700
Plasmodium berghei PBANKA_0803100   DNA replication licensing factor MCM7, putative
Plasmodium falciparum PF3D7_0705400   DNA replication licensing factor MCM7
Plasmodium knowlesi PKNH_0103900   DNA replication licensing factor MCM7, putative
Plasmodium vivax PVX_087810   DNA replication licensing factor MCM7, putative
Plasmodium yoelii PY03236   DNA replication licensing factor mcm7
Saccharomyces cerevisiae YBR202W   mini-chromosome maintenance complex protein 7
Schistosoma japonicum Sjp_0112300   ko:K02210 minichromosome maintenance protein 7 (cell division control protein, putative
Schistosoma japonicum Sjp_0096330   DNA replication licensing factor mcm7-B, putative
Schistosoma japonicum Sjp_0051550   DNA replication licensing factor mcm7-A, putative
Schistosoma mansoni Smp_032500   DNA replication licensing factor MCM7
Schmidtea mediterranea mk4.033904.00   DNA replication licensing factor MCM7
Schmidtea mediterranea mk4.029139.00   DNA replication licensing factor MCM7
Schmidtea mediterranea mk4.079970.00   DNA replication licensing factor MCM7
Trypanosoma brucei gambiense Tbg972.11.18100   minichromosome maintenance (MCM) complex subunit, putative
Trypanosoma brucei Tb927.11.16140   DNA replication licensing factor MCM7
Trypanosoma cruzi TcCLB.508707.210   DNA replication licensing factor MCM7, putative
Toxoplasma gondii TGME49_237220   DNA replication licensing factor Mcm7, putative
Theileria parva TP03_0236   DNA replication licensing factor MCM7, putative
Trichomonas vaginalis TVAG_130190   DNA replication licensing factor, putative

Essentiality

PF3D7_0705400 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.7810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.7810 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.7810 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.7810 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F32D1.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_F32D1.10 Caenorhabditis elegans sterile wormbase
YBR202W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0803100 Plasmodium berghei Essential plasmo
TGME49_237220 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0705400 (Plasmodium falciparum), DNA replication licensing factor MCM7
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