Detailed view for PBANKA_0933700

Basic information

TDR Targets ID: 826630
Plasmodium berghei, mitogen-activated protein kinase 2
Source Database / ID: 

pI: 7.4979 | Length (AA): 523 | MW (Da): 61122 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004707   MAP kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 508 1ym7 (A) 76 526 19.00 0 1 0.843119 0.9
12 102 2y8d (A) 2520 2625 18.00 0 0 0.300796 -1.26
110 519 3n9x (A) 19 428 99.99 0 1 1.82154 -0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Female gametocyte. Yeoh LM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Gametocyte, 22 hs Schizont. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Erthyrocytic stages, Male gametocyte. Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile 4 hs Ring, 16 hs Trophozoite. Otto TD
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Ookinete. Otto TD
Show/Hide expression data references
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.

Orthologs

Ortholog group members (OG5_138034)

Species Accession Gene Product
Babesia bovis BBOV_IV005520   mitogen-activated protein kinase, putative
Cryptosporidium hominis Chro.20465   mitogen-activated protein kinase 2
Cryptosporidium parvum cgd2_4340   mitogen-activated protein kinase 2, putative
Neospora caninum NCLIV_002760   CMGC kinase, MAPK family, putative
Plasmodium berghei PBANKA_0933700   mitogen-activated protein kinase 2
Plasmodium falciparum PF3D7_1113900   mitogen-activated protein kinase 2
Plasmodium knowlesi PKNH_0911500   mitogen-activated protein kinase 2, putative
Plasmodium vivax PVX_091340   mitogen-activated protein kinase 2, putative
Plasmodium yoelii PY00731   mitogen-activated protein kinase 2
Toxoplasma gondii TGME49_207820   cell-cycle-associated protein kinase MAPK, putative
Theileria parva TP01_0359   mitogen-activated protein kinase 2, putative

Essentiality

PBANKA_0933700 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0933700 this record Plasmodium berghei Dispensable plasmo
TGME49_207820 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum mitogen-activated protein kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus MAP kinase p38 alpha 360 aa 34.6% 344 aa Compounds References
Sus scrofa Glycogen synthase kinase 3 beta 420 aa 30.5% 351 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 24.9% 277 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 34.5% 200 aa Compounds References
Patiria pectinifera Cdc2 300 aa 31.8% 336 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 35.5% 335 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 37.6% 378 aa Compounds References
Rattus norvegicus Glycogen synthase kinase-3 beta 420 aa 29.9% 355 aa Compounds References
Bos taurus Glycogen synthase kinase-3 beta splice variant X1 419 aa 30.5% 351 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 31.7% 338 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 25.7% 358 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 8 411 aa 31.6% 345 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 21.5% 344 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 34.3% 335 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 21.8% 344 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PBANKA_0933700 (Plasmodium berghei), mitogen-activated protein kinase 2
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