Detailed view for PBANKA_1126900

Basic information

TDR Targets ID: 827185
Plasmodium berghei, eukaryotic translation initiation factor 2-alpha kinase
Source Database / ID: 

pI: 9.4885 | Length (AA): 2591 | MW (Da): 306873 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
112 1335 5ng6 (G) 12 1231 18.00 0 1 0.233104 1.42
2226 2529 1luf (A) 563 860 17.00 0 0.93 0.138529 -0.06
2237 2555 3c0g (B) 12 304 15.00 0 0.67 0.0973185 0.25
2243 2557 4hzs (A) 132 414 19.00 0 1 0.0297747 0.74
2336 2393 3gbz (A) 101 164 45.00 0.98 0.93 0.487985 -0.02
2352 2395 4ic8 (A) 143 200 51.00 0.84 0.48 0.385582 1.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile 16 hs Trophozoite. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile 4 hs Ring, Erthyrocytic stages, Female gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Ookinete, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Gametocyte, 22 hs Schizont. Otto TD
Show/Hide expression data references
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.

Orthologs

Ortholog group members (OG5_140247)

Species Accession Gene Product
Babesia bovis BBOV_IV011370   PK4 protein kinase
Cryptosporidium hominis Chro.10109   protein kinase domain
Cryptosporidium parvum cgd1_890   protein kinase domain
Dictyostelium discoideum DDB_G0276043   GCN2 subfamily protein kinase
Dictyostelium discoideum DDB_G0268642   PEK family protein kinase
Dictyostelium discoideum DDB_G0295843   hypothetical protein
Plasmodium berghei PBANKA_1126900   eukaryotic translation initiation factor 2-alpha kinase
Plasmodium falciparum PF3D7_0628200   eukaryotic translation initiation factor 2-alpha kinase
Plasmodium knowlesi PKNH_1121600   eukaryotic translation initiation factor 2-alpha kinase, putative
Plasmodium vivax PVX_114512   eukaryotic translation initiation factor 2-alpha kinase, putative
Plasmodium yoelii PY05545   Protein kinase domain, putative
Theileria parva TP01_1167   protein kinase, putative

Essentiality

PBANKA_1126900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1126900 this record Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PBANKA_1126900 (Plasmodium berghei), eukaryotic translation initiation factor 2-alpha kinase
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