Detailed view for PBANKA_0722000

Basic information

TDR Targets ID: 828846
Plasmodium berghei, phosphatidylinositol 4-kinase, putative
Source Database / ID: 

pI: 9.978 | Length (AA): 4625 | MW (Da): 557860 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 17

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0016301   kinase activity  
GO:0048015   phosphoinositide-mediated signaling  
GO:0046854   phosphoinositide phosphorylation  

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
284 1117 3vuo (A) 358 1184 24.00 0.00034 0.77 0.0474243 2.03
595 1633 5wtj (A) 403 1363 27.00 0.0000000094 1 0.121749 2.16
872 1724 1z3h (A) 17 935 12.00 0 0.98 0.0580324 0.75
1894 3014 5xus (A) 28 1111 19.00 0 1 -0.125022 1.83
3875 4065 4uos (A) 1 183 12.00 0.0053 0.02 0.148897 -0.58
4313 4606 5c4g (E) 390 782 22.00 0 1 0.209168 0.52
4414 4609 3ls8 (B) 677 870 25.00 0 1 0.101978 0.1
4420 4607 5anl (A) 682 868 24.00 0 0.98 0.122249 -0.1
4438 4602 4jsn (B) 2298 2504 31.00 0.068 0.98 0.213776 0.93

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Ookinete, 16 hs Trophozoite, Erthyrocytic stages, Female gametocyte, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Gametocyte, 4 hs Ring, 22 hs Schizont. Otto TD
Show/Hide expression data references
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.

Orthologs

Ortholog group members (OG5_165972)

Species Accession Gene Product
Plasmodium berghei PBANKA_0722000   phosphatidylinositol 4-kinase, putative
Plasmodium falciparum PF3D7_0419900   phosphatidylinositol 4-kinase, putative
Plasmodium knowlesi PKNH_0512600   phosphatidylinositol 4-kinase, putative
Plasmodium vivax PVX_090085   phosphatidylinositol 4-kinase, putative
Plasmodium yoelii PY06820   hypothetical protein

Essentiality

PBANKA_0722000 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0722000 this record Plasmodium berghei Slow plasmo
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PBANKA_0722000 (Plasmodium berghei), phosphatidylinositol 4-kinase, putative
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