Detailed view for PF3D7_0823600

Basic information

TDR Targets ID: 829616
Plasmodium falciparum, lipoate-protein ligase B
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 10.0962 | Length (AA): 434 | MW (Da): 51638 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0033819   lipoyl(octanoyl) transferase activity  
GO:0009249   protein lipoylation  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
73 424 2qhs (A) 2 210 25.00 0 1 0.33896 1.34
217 255 1w66 (A) 70 114 51.00 0.44 0.15 0.306962 1.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Ring. Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 48 hs, Oocyst, Sporozoite. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, Female Gametocyte, Male Gametocyte. Otto TD Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127515)

Species Accession Gene Product
Arabidopsis thaliana AT4G31050   Biotin/lipoate A/B protein ligase family
Arabidopsis thaliana AT1G47578   putative lipoyltransferase-like protein
Arabidopsis thaliana AT1G04640   lipoyltransferase 2
Brugia malayi Bm1_52715   lipoate-protein ligase B containing protein
Candida albicans CaO19.10528   similar to E. coli LipB protein
Candida albicans CaO19.3010   similar to E. coli LipB protein
Dictyostelium discoideum DDB_G0275417   hypothetical protein
Drosophila melanogaster Dmel_CG9804   CG9804 gene product from transcript CG9804-RA
Escherichia coli b0630   octanoyltransferase
Echinococcus granulosus EgrG_000444900   octanoyltransferase mitochondrial
Echinococcus multilocularis EmuJ_000444900   octanoyltransferase, mitochondrial
Homo sapiens ENSG00000175536   lipoyl(octanoyl) transferase 2 (putative)
Leishmania braziliensis LbrM.35.3300   lipoate protein ligase, putative
Leishmania donovani LdBPK_363230.1   Octanoyltransferase, putative
Leishmania infantum LinJ.36.3230   lipoate protein ligase, putative
Leishmania major LmjF.36.3080   lipoate protein ligase, putative
Leishmania mexicana LmxM.36.3080   lipoate protein ligase, putative
Loa Loa (eye worm) LOAG_00038   hypothetical protein
Mycobacterium leprae ML0859c   Probable lipoate biosynthesis protein B LipB
Mus musculus 67164   lipoyl(octanoyl) transferase 2 (putative)
Mycobacterium tuberculosis Rv2217   Probable lipoate biosynthesis protein B LipB
Mycobacterium ulcerans MUL_1344   lipoate-protein ligase B
Neospora caninum NCLIV_058320   Strongly similar to lipoyltransferase, related
Oryza sativa 4332681   Os03g0321800
Oryza sativa 4352692   Os12g0596000
Onchocerca volvulus OVOC6679   Putative lipoyltransferase 2, mitochondrial
Plasmodium berghei PBANKA_0707000   lipoate-protein ligase B
Plasmodium falciparum PF3D7_0823600   lipoate-protein ligase B
Plasmodium knowlesi PKNH_1317600   lipoate-protein ligase B, putative
Plasmodium vivax PVX_089180   lipoate-protein ligase B, putative
Plasmodium yoelii PY04501   putative ligase in lipoate biosynthesis
Saccharomyces cerevisiae YLR239C   lipoyl(octanoyl) transferase LIP2
Schistosoma japonicum Sjp_0218640   expressed protein
Schistosoma japonicum Sjp_0114740   ko:K03801 lipoyl(octanoyl) transferase, putative
Schistosoma mansoni Smp_176550   lipoate-protein ligase B
Schmidtea mediterranea mk4.005587.00   Putative lipoyltransferase 2, mitochondrial
Schmidtea mediterranea mk4.058520.02   Putative lipoyltransferase 2, mitochondrial
Trypanosoma brucei gambiense Tbg972.11.10520   lipoate-protein ligase, putative,lipoyltransferase, putative
Trypanosoma brucei Tb927.11.9390   Octanoyltransferase, putative
Trypanosoma congolense TcIL3000.11.9800   Octanoyltransferase, putative
Trypanosoma cruzi TcCLB.511587.120   Octanoyltransferase, putative
Trypanosoma cruzi TcCLB.508851.40   Octanoyltransferase, putative
Toxoplasma gondii TGME49_315640   lipoyl(octanoyl) transferase
Wolbachia endosymbiont of Brugia malayi Wbm0235   lipoate-protein ligase B

Essentiality

PF3D7_0823600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.1160 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.1160 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.1160 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.1160 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0630 Escherichia coli non-essential goodall
PBANKA_0707000 Plasmodium berghei Dispensable plasmo
TGME49_315640 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0823600 (Plasmodium falciparum), lipoate-protein ligase B
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