Detailed view for PF3D7_1404600

Basic information

TDR Targets ID: 829835
Plasmodium falciparum, adenylyl cyclase alpha
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.3753 | Length (AA): 803 | MW (Da): 96604 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 4

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00211   Adenylate and Guanylate cyclase catalytic domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0070483   GO:detection of hypoxia  

GO:0046777   protein amino acid autophosphorylation  
GO:0035556   GO:intracellular signal transduction  

GO:0016849   phosphorus-oxygen lyase activity  
GO:0004673   protein histidine kinase activity  
GO:0009190   cyclic nucleotide biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
516 724 3r5g (A) 232 406 30.00 0.000069 1 0.427018 0.05
516 721 1wc3 (A) 1015 1190 31.00 0.0079 0.86 0.18355 0.71
656 864 4tql (A) 23 228 15.00 0.97 0.17 0.327318 -0.28
312 531 1wc3 (A) 1007 1196 23.00 0.0000022 1 0.479349 -0.23
316 531 1fx4 (A) 888 1092 14.00 0.47 0.99 0.49337 -0.44
320 525 1wc3 (A) 1015 1190 31.00 0.0062 0.86 0.252922 0.71
442 665 1wc3 (A) 1003 1196 21.00 0.093 0.84 0.365854 0.08
454 662 3r5g (A) 232 406 30.00 0.000068 1 0.446774 0.05
454 659 1wc3 (A) 1015 1190 31.00 0.0077 0.86 0.203038 0.71
616 795 4tql (A) 46 239 19.00 0.9 0.28 0.333059 -0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Female Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 48 hs, Oocyst, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs. Otto TD
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_134515)

Species Accession Gene Product
Cryptosporidium hominis Chro.20139   hypothetical protein
Cryptosporidium parvum cgd2_1270   membrane associated adenylyl cyclase with 6 transmembrane regions and an adenylyl cyclase domain
Neospora caninum NCLIV_041540   adenylyl cyclase, putative
Plasmodium berghei PBANKA_1037500   adenylyl cyclase alpha
Plasmodium falciparum PF3D7_1404600   adenylyl cyclase alpha
Plasmodium knowlesi PKNH_1353500   adenylyl cyclase alpha, putative
Plasmodium yoelii PY00993   hypothetical protein
Toxoplasma gondii TGME49_289170   adenylate and guanylate cyclase catalytic domain-containing protein

Essentiality

PF3D7_1404600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_289170 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1404600 (Plasmodium falciparum), adenylyl cyclase alpha
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