Detailed view for TGME49_212710

Basic information

TDR Targets ID: 835371
Toxoplasma gondii, phenylalanine-4-hydroxylase
Source Database / ID:  ToxoDB 

pI: 7.0063 | Length (AA): 569 | MW (Da): 62944 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00351   Biopterin-dependent aromatic amino acid hydroxylase
PF01842   ACT domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016714   oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen  
GO:0005506   iron ion binding  
GO:0004497   monooxygenase activity  
GO:0055114   oxidation reduction  
GO:0009072   aromatic amino acid family metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
54 301 1yx1 (A) 11 248 28.00 0.28 0.85 0.550952 0.59
160 569 1phz (A) 20 426 39.00 0 1 1.25796 -1.03
254 569 1toh (A) 164 472 52.00 0 1 1.23646 -1.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_131392)

Species Accession Gene Product
Caenorhabditis elegans CELE_K08F8.4   Protein PAH-1, isoform A
Dictyostelium discoideum DDB_G0278781   phenylalanine 4-monooxygenase
Drosophila melanogaster Dmel_CG7399   Henna
Homo sapiens ENSG00000171759   phenylalanine hydroxylase
Leishmania braziliensis LbrM.28.1380   phenylalanine-4-hydroxylase, putative;with=GeneDB:LmjF28.1280
Leishmania donovani LdBPK_281390.1   phenylalanine-4-hydroxylase, putative
Leishmania infantum LinJ.28.1390   phenylalanine-4-hydroxylase, putative
Leishmania major LmjF.28.1280   phenylalanine-4-hydroxylase,phenylalanine-4-hydroxylase, putative
Leishmania mexicana LmxM.28.1280   phenylalanine-4-hydroxylase, putative
Mus musculus ENSMUSG00000020051   phenylalanine hydroxylase
Neospora caninum NCLIV_069680   hypothetical protein
Toxoplasma gondii TGME49_287510   aromatic amino acid hydrolase AAH1
Toxoplasma gondii TGME49_212710   phenylalanine-4-hydroxylase
Toxoplasma gondii TGME49_212740   aromatic amino acid hydrolase AAH2

Essentiality

TGME49_212710 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_K08F8.4 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Phenylalanine-4-hydroxylase Compounds References
Homo sapiens phenylalanine hydroxylase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Tryptophan 5-monooxygenase 1 444 aa 41.1% 399 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0129 0.259 1
0.0253 0.2667 1
0.0139 0.2562 1
0.0137 0.2579 1
0.0243 0.6652 1
0.0375 0.5513 1
0.0253 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_212710 (Toxoplasma gondii), phenylalanine-4-hydroxylase
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