Detailed view for Tb11.v5.0176

Basic information

TDR Targets ID: 931500
Trypanosoma brucei, NAD(p)-dependent steroid dehydrogenase-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3855 | Length (AA): 442 | MW (Da): 49530 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01073   3-beta hydroxysteroid dehydrogenase/isomerase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016616   oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor  
GO:0003854   3-beta-hydroxy-delta5-steroid dehydrogenase activity  
GO:0006694   steroid biosynthetic process  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128811)

Species Accession Gene Product
Arabidopsis thaliana AT1G47290   3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Arabidopsis thaliana AT2G26260   3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Candida albicans CaO19.10427   C-3 sterol dehydrogenase/C-4 decarboxylase
Candida albicans CaO19.2909   C-3 sterol dehydrogenase/C-4 decarboxylase
Caenorhabditis elegans CELE_ZC8.1   Protein HSD-2
Dictyostelium discoideum DDB_G0281487   3beta-hydroxysteroid dehydrogenase
Escherichia coli b0868   putative NAD(P)H-dependent oxidoreductase with NAD(P)-binding Rossmann-fold domain
Homo sapiens ENSG00000147383   NAD(P) dependent steroid dehydrogenase-like
Leishmania braziliensis LbrM.06.0330   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania donovani LdBPK_060350.1   NAD(P)-dependent steroid dehydrogenase protein, putative
Leishmania infantum LinJ.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania major LmjF.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania mexicana LmxM.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Loa Loa (eye worm) LOAG_09025   hypothetical protein
Mycobacterium leprae ML1942   PROBABLE CHOLESTEROL DEHYDROGENASE
Mus musculus ENSMUSG00000031349   NAD(P) dependent steroid dehydrogenase-like
Oryza sativa 4333071   Os03g0405000
Saccharomyces cerevisiae YGL001C   sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating)
Trypanosoma brucei gambiense Tbg972.7.5680   NAD(p)-dependent steroid dehydrogenase-like protein
Trypanosoma brucei Tb927.7.4950   NAD(P)-dependent steroid dehydrogenase protein, putative
Trypanosoma brucei Tb11.v5.0176   NAD(p)-dependent steroid dehydrogenase-like protein
Trypanosoma congolense TcIL3000_0_27580   NAD(P)-dependent steroid dehydrogenase protein, putative

Essentiality

Tb11.v5.0176 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4950 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.4950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0868 Escherichia coli non-essential goodall
YGL001C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Mycobacterium tuberculosis 3-beta-hydroxysteroid dehydrogenase 370 aa 31.0% 355 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb11.v5.0176 (Trypanosoma brucei), NAD(p)-dependent steroid dehydrogenase-like protein
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