Detailed view for LOAG_09025

Basic information

TDR Targets ID: 935375
Loa Loa (eye worm), hypothetical protein
Source Database / ID:  KEGG  

pI: 8.8082 | Length (AA): 392 | MW (Da): 44479 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01073   3-beta hydroxysteroid dehydrogenase/isomerase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016616   oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor  
GO:0003854   3-beta-hydroxy-delta5-steroid dehydrogenase activity  
GO:0055114   oxidation reduction  
GO:0006694   steroid biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 341 4r1s (A) 4 317 18.00 0 1 0.916898 0.35
5 79 3e48 (A) 2 75 11.00 0 0.17 0.259227 0.22
7 341 3wj7 (A) 14 337 25.00 0 1 1.10259 0.34
7 109 3tl3 (A) 9 123 20.00 0.0013 0.66 0.398755 -0.38
8 43 5nc8 (A) 410 445 47.00 0.78 0.52 0.479137 1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128811)

Species Accession Gene Product
Arabidopsis thaliana AT1G47290   3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Arabidopsis thaliana AT2G26260   3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
Candida albicans CaO19.10427   C-3 sterol dehydrogenase/C-4 decarboxylase
Candida albicans CaO19.2909   C-3 sterol dehydrogenase/C-4 decarboxylase
Caenorhabditis elegans CELE_ZC8.1   Protein HSD-2
Dictyostelium discoideum DDB_G0281487   3beta-hydroxysteroid dehydrogenase
Escherichia coli b0868   putative NAD(P)H-dependent oxidoreductase with NAD(P)-binding Rossmann-fold domain
Homo sapiens ENSG00000147383   NAD(P) dependent steroid dehydrogenase-like
Leishmania braziliensis LbrM.06.0330   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania donovani LdBPK_060350.1   NAD(P)-dependent steroid dehydrogenase protein, putative
Leishmania infantum LinJ.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania major LmjF.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Leishmania mexicana LmxM.06.0350   NAD(p)-dependent steroid dehydrogenase-like protein
Loa Loa (eye worm) LOAG_09025   hypothetical protein
Mycobacterium leprae ML1942   PROBABLE CHOLESTEROL DEHYDROGENASE
Mus musculus ENSMUSG00000031349   NAD(P) dependent steroid dehydrogenase-like
Oryza sativa 4333071   Os03g0405000
Saccharomyces cerevisiae YGL001C   sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating)
Trypanosoma brucei gambiense Tbg972.7.5680   NAD(p)-dependent steroid dehydrogenase-like protein
Trypanosoma brucei Tb927.7.4950   NAD(P)-dependent steroid dehydrogenase protein, putative
Trypanosoma brucei Tb11.v5.0176   NAD(p)-dependent steroid dehydrogenase-like protein
Trypanosoma congolense TcIL3000_0_27580   NAD(P)-dependent steroid dehydrogenase protein, putative

Essentiality

LOAG_09025 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4950 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.4950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.4950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0868 Escherichia coli non-essential goodall
YGL001C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Mycobacterium tuberculosis 3-beta-hydroxysteroid dehydrogenase 370 aa 29.1% 344 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LOAG_09025 (Loa Loa (eye worm)), hypothetical protein
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