Detailed view for LOAG_14342

Basic information

TDR Targets ID: 935466
Loa Loa (eye worm), phosphatidylinositol-specific phospholipase C
Source Database / ID:  KEGG  

pI: 8.2729 | Length (AA): 286 | MW (Da): 32886 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00388   Phosphatidylinositol-specific phospholipase C, X domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008081   phosphoric diester hydrolase activity  
GO:0006629   lipid metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 192 4i90 (A) 8 175 20.00 0 0.98 0.718529 0.45
2 283 2plc (A) 24 279 19.00 0 0.96 1.09021 0.16
63 184 3h4x (A) 40 195 16.00 0 0.43 0.311773 0.12
154 217 4kp1 (A) 37 87 39.00 0.28 0.17 0.296876 0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130000)

Species Accession Gene Product
Brugia malayi Bm1_57135   Phosphatidylinositol-specific phospholipase C, X domain containing protein
Brugia malayi Bm1_36730   MGC53544 protein
Caenorhabditis elegans CELE_F38E9.1   Protein F38E9.1
Dictyostelium discoideum DDB_G0267488   hypothetical protein
Dictyostelium discoideum DDB_G0293730   TIM beta/alpha-barrel domain-containing protein
Dictyostelium discoideum DDB_G0269228   phosphatidylinositol-specific phospholipase C X domain-containing protein
Drosophila melanogaster Dmel_CG10747   CG10747 gene product from transcript CG10747-RB
Echinococcus granulosus EgrG_000999500   PLC phosphodiesterase TIM beta alpha barrel domain
Echinococcus granulosus EgrG_001071600   Phospholipase C
Entamoeba histolytica EHI_012410   hypothetical protein
Entamoeba histolytica EHI_049280   hypothetical protein
Entamoeba histolytica EHI_103490   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000999500   PLC phosphodiesterase, TIM beta alpha barrel domain
Echinococcus multilocularis EmuJ_001071600   Phospholipase C
Homo sapiens ENSG00000240891   phosphatidylinositol-specific phospholipase C, X domain containing 2
Homo sapiens ENSG00000182836   phosphatidylinositol-specific phospholipase C, X domain containing 3
Loa Loa (eye worm) LOAG_14342   phosphatidylinositol-specific phospholipase C
Loa Loa (eye worm) LOAG_05941   hypothetical protein
Mus musculus ENSMUSG00000049148   phosphatidylinositol-specific phospholipase C, X domain containing 3
Mus musculus ENSMUSG00000087141   phosphatidylinositol-specific phospholipase C, X domain containing 2
Onchocerca volvulus OVOC9286  
Schistosoma japonicum Sjp_0026320   ko:K01114 phospholipase C [EC3.1.4.3], putative
Schistosoma mansoni Smp_018360   hypothetical protein
Schmidtea mediterranea mk4.029690.00  
Schmidtea mediterranea mk4.000258.07  
Schmidtea mediterranea mk4.004696.00  
Schmidtea mediterranea mk4.001510.02  
Schmidtea mediterranea mk4.000819.02  
Schmidtea mediterranea mk4.001510.03  
Trypanosoma brucei gambiense Tbg.972.2.4210   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma brucei Tb927.2.6000   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma congolense TcIL3000_2_1660   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.508765.30   VSG lipase
Trypanosoma cruzi TcCLB.506821.30   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.508807.5   VSG lipase
Trypanosoma cruzi TcCLB.508233.50   vsglipase, putative
Trypanosoma cruzi TcCLB.506617.70   glycosylphosphatidylinositol-specific phospholipase C
Trypanosoma cruzi TcCLB.507041.120   variant-surface-glycoprotein phospholipase C, putative

Essentiality

LOAG_14342 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.6000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.6000 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.2.6000 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.6000 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LOAG_14342 (Loa Loa (eye worm)), phosphatidylinositol-specific phospholipase C
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