Detailed view for LOAG_06201

Basic information

TDR Targets ID: 939451
Loa Loa (eye worm), WNK protein kinase
Source Database / ID:  KEGG  

pI: 5.5723 | Length (AA): 1406 | MW (Da): 155393 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF12202   Oxidative-stress-responsive kinase 1 C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 451 2fo0 (A) 84 528 23.00 0 1 0.439667 0.51
10 440 2c0t (A) 61 498 26.00 0 1 0.432843 0.4
49 436 5xzw (B) 66 466 28.00 0 1 0.42426 0.68
101 492 5uvc (A) 88 528 21.00 0 1 0.318105 0.35
111 424 4wb7 (A) 41 335 28.00 0.000000000069 1 0.487029 -0.27
158 519 5o2c (A) 131 496 63.00 0 1 0.992768 -0.69
174 726 4kik (A) 15 637 22.00 0 1 0.463614 0.66
438 521 2lru (A) 479 572 39.00 0.00000014 1 0.529444 -1.23
626 691 5g3q (A) 1120 1186 32.00 0.58 1 0.604642 -1.81
703 820 2mqa (A) 35 138 35.00 0.76 0.14 0.298226 -0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127736)

Species Accession Gene Product
Arabidopsis thaliana AT1G64630   putative serine/threonine-protein kinase WNK10
Arabidopsis thaliana AT1G49160   putative serine/threonine-protein kinase WNK7
Arabidopsis thaliana AT5G41990   serine/threonine-protein kinase WNK8
Arabidopsis thaliana AT3G48260   putative serine/threonine-protein kinase WNK3
Brugia malayi Bm1_22880   Protein kinase domain containing protein
Caenorhabditis elegans CELE_C46C2.1   Protein WNK-1, isoform L
Drosophila melanogaster Dmel_CG7177   WNK homolog
Echinococcus granulosus EgrG_000259100   serine:threonine protein kinase WNK1
Echinococcus granulosus EgrG_002005000   5'partial|protein kinase
Echinococcus multilocularis EmuJ_000017500   protein kinase
Echinococcus multilocularis EmuJ_000259100   serine:threonine protein kinase WNK1
Giardia lamblia GL50803_90343   Kinase, Wnk
Homo sapiens ENSG00000126562   WNK lysine deficient protein kinase 4
Homo sapiens ENSG00000196632   WNK lysine deficient protein kinase 3
Homo sapiens ENSG00000060237   WNK lysine deficient protein kinase 1
Homo sapiens ENSG00000165238   WNK lysine deficient protein kinase 2
Loa Loa (eye worm) LOAG_12245   hypothetical protein
Loa Loa (eye worm) LOAG_06201   WNK protein kinase
Mus musculus ENSMUSG00000037989   WNK lysine deficient protein kinase 2
Mus musculus ENSMUSG00000035112   WNK lysine deficient protein kinase 4
Mus musculus ENSMUSG00000041245   WNK lysine deficient protein kinase 3
Mus musculus ENSMUSG00000045962   WNK lysine deficient protein kinase 1
Oryza sativa 4342585   Os07g0185000
Oryza sativa 4330286   Os02g0672800
Oryza sativa 4337579   Os05g0108300
Schistosoma japonicum Sjp_0040710   ko:K08867 WNK lysine deficient protein kinase, putative
Schistosoma japonicum Sjp_0040670   Methionine-R-sulfoxide reductase B3, mitochondrial precursor, putative
Schistosoma mansoni Smp_154440   protein kinase
Schmidtea mediterranea mk4.000805.03   Protein kinase
Schmidtea mediterranea mk4.002865.03  
Schmidtea mediterranea mk4.002865.00  
Schmidtea mediterranea mk4.026761.00   WNK homolog
Schmidtea mediterranea mk4.000281.01  
Schmidtea mediterranea mk4.002865.04   WNK homolog
Trichomonas vaginalis TVAG_388240   STE family protein kinase

Essentiality

LOAG_06201 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_C46C2.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens WNK lysine deficient protein kinase 3 Compounds References
Homo sapiens WNK lysine deficient protein kinase 1 Compounds References
Homo sapiens WNK lysine deficient protein kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.8% 160 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.3% 270 aa Compounds References
Sus scrofa Casein kinase I isoform alpha 125 aa 28.0% 107 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 29.3% 205 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0018 0.5 0.5
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0042 0.5 0.5
0.0069 0.3067 0.9179
0.0063 0.7244 0.6686
0.0027 1 1
0.0197 0.2503 0.2994
0.0197 0.2503 0.2994
0.0171 0.2659 0.325
0.0059 1 1
0.0061 0.6883 0.6883
0.0193 0.2615 0.3203
0.0092 1 1
0.0026 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0081 1 1
0.0093 0.8828 0.8828
0.0012 0.5 0.5
0.0062 0.6935 0.6935
0.0016 0.5 0.5
0.0197 0.2503 0.2994
0.0032 0.5 0.5
0.0022 0.5 0.5
0.0081 0.5 0.5
0.0029 0.5 0.5
0.0003 0.5 0.5
0.0039 0.9485 1
0.0187 0.426 0.508
0.0064 0.3377 0.3377
0.0037 1 0.5
0.0185 0.4376 0.5108
0.0088 0.4477 0.4477
0.0007 0.5 0.5
0.0059 1 1
0.0158 0.4323 0.5106
0.0066 0.3101 0.3068
0.0063 1 1
0.0091 1 1
0.0011 1 1
0.0162 0.4519 0.4519
0.0518 0.3928 0.3917
0.0056 1 1
0.0059 1 1
0.0067 0.5 0.5
0.0033 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0098 0.3242 0.3017
0.0032 0.5 0.5
0.0184 0.4335 0.5098
0.0008 0.5 0.5
0.0023 0.5 0.5
0.0012 0.5 0.5
0.0004 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LOAG_06201 (Loa Loa (eye worm)), WNK protein kinase
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