Detailed view for LOAG_11954

Basic information

TDR Targets ID: 944035
Loa Loa (eye worm), STE/STE7/MEK4 protein kinase
Source Database / ID:  KEGG  

pI: 6.9504 | Length (AA): 310 | MW (Da): 35177 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

MAPK signaling pathway (KEGG)
ErbB signaling pathway (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 305 3aln (A) 94 388 50.00 0 1 1.28179 0
10 302 3enm (A) 51 332 47.00 0 1 1.37276 -0.6

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_144825)

Species Accession Gene Product
Brugia malayi Bm1_54585   Protein kinase domain containing protein
Echinococcus granulosus EgrG_000221400   dual specificity mitogen activated protein
Echinococcus multilocularis EmuJ_000221400   dual specificity mitogen activated protein
Leishmania braziliensis LbrM.24.2400   mitogen-activated protein kinase
Leishmania donovani LdBPK_242410.1   mitogen-activated protein kinase
Leishmania infantum LinJ.24.2410   mitogen-activated protein kinase
Leishmania major LmjF.24.2320   mitogen-activated protein kinase
Leishmania mexicana LmxM.24.2320   mitogen-activated protein kinase
Loa Loa (eye worm) LOAG_11954   STE/STE7/MEK4 protein kinase
Schistosoma japonicum Sjp_0034990   Dual specificity mitogen-activated protein kinase kinase 6, putative
Schistosoma japonicum Sjp_0084080   Dual specificity mitogen-activated protein kinase kinase 6, putative
Schistosoma mansoni Smp_073510   protein kinase
Schmidtea mediterranea mk4.015537.00  
Schmidtea mediterranea mk4.023281.00  
Trypanosoma brucei gambiense Tbg972.8.5950   protein kinase, putative
Trypanosoma brucei Tb927.8.5950   mitogen-activated protein kinase kinase 4, putative
Trypanosoma congolense TcIL3000_8_5700   protein kinase, putative
Trypanosoma cruzi TcCLB.440099.28   protein kinase, putative
Trypanosoma cruzi TcCLB.511075.60   mitogen-activated protein kinase kinase 4, putative

Essentiality

LOAG_11954 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5950 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5950 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5950 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0036 0.5 0.5
0.0067 0.5 0.5
0.0008 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0061 0.6883 0.6883
0.0007 0.5 0.5
0.0062 0.6935 0.6935
0.0012 0.5 0.5
0.0081 0.5 0.5
0.0033 0.5 0.5
0.0003 0.5 0.5
0.0011 1 1
0.0018 0.5 0.5
0.0092 1 1
0.0004 0.5 0.5
0.0026 0.5 0.5
0.0066 0.3101 0.3068
0.0042 0.5 0.5
0.0007 0.5 0.5
0.0069 0.3067 0.9179
0.0029 0.5 0.5
0.0016 0.5 0.5
0.0023 0.5 0.5
0.0037 1 0.5
0.0088 0.4477 0.4477
0.0063 0.7244 0.6686
0.0059 1 1
0.0056 1 1
0.0033 1 1
0.0091 1 1
0.0064 0.3377 0.3377
0.0022 0.5 0.5
0.0081 1 1
0.0039 0.5 0.5
0.0027 1 1
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0063 1 1
0.0093 0.8828 0.8828
0.0032 0.5 0.5
0.0016 0.5 0.5
0.0039 0.9485 1
0.0059 1 1
0.0039 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.3017

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier LOAG_11954 (Loa Loa (eye worm)), STE/STE7/MEK4 protein kinase
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