Detailed view for MUL_1443

Basic information

TDR Targets ID: 954260
Mycobacterium ulcerans, n-acetylglucosamine-6-phosphate deacetylase NagA
Source Database / ID:  KEGG  

pI: 5.8364 | Length (AA): 388 | MW (Da): 39294 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01979   Amidohydrolase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016810   hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds  
GO:0016787   hydrolase activity  
GO:0008448   N-acetylglucosamine-6-phosphate deacetylase activity  
GO:0006044   N-acetylglucosamine metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 383 6fv4 (A) 1 375 48.00 0 1 1.57834 -0.72
3 384 6fv3 (D) 2 376 49.00 0 1 1.48964 -0.34
44 382 1o12 (A) 37 358 35.00 0 1 1.29781 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128970)

Species Accession Gene Product
Candida albicans CaO19.9704   predicted N-acetylglucosamine-6-phosphate deacetylase, similar to bacterial NagA
Candida albicans CaO19.2157   predicted N-acetylglucosamine-6-phosphate deacetylase, similar to bacterial NagA
Caenorhabditis elegans CELE_F59B2.3   Protein F59B2.3
Drosophila melanogaster Dmel_CG17065   CG17065 gene product from transcript CG17065-RD
Escherichia coli b0677   N-acetylglucosamine-6-phosphate deacetylase
Homo sapiens ENSG00000162066   amidohydrolase domain containing 2
Mus musculus ENSMUSG00000036820   amidohydrolase domain containing 2
Mycobacterium tuberculosis Rv3332   Probable N-acetylglucosamine-6-phosphate deacetylase NagA (GlcNAc 6-P deacetylase)
Mycobacterium ulcerans MUL_1443   n-acetylglucosamine-6-phosphate deacetylase NagA
Schistosoma japonicum Sjp_0064410   ko:K01443 N-acetylglucosamine-6-phosphate deacetylase [EC3.5.1.25], putative
Schistosoma mansoni Smp_033660   hypothetical protein
Schmidtea mediterranea mk4.059399.00   Putative N-acetylglucosamine-6-phosphate deacetylase

Essentiality

MUL_1443 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0677 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens amidohydrolase domain containing 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier MUL_1443 (Mycobacterium ulcerans), n-acetylglucosamine-6-phosphate deacetylase NagA
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