Detailed view for Sjp_0004230

Basic information

TDR Targets ID: 975283
Schistosoma japonicum, ko:K06641 serine/threonine-protein kinase Chk2, putative
Source Database / ID:  Wormbase Parasite  

pI: 4.8479 | Length (AA): 1186 | MW (Da): 134716 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00400   WD domain, G-beta repeat
PF00498   FHA domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
158 683 5i2t (A) 16 593 20.00 0 1 0.395082 1.23
480 618 5m89 (A) 276 395 20.00 0.000015 1 0.2455 -0.34
540 614 5k19 (A) 230 290 38.00 0.48 0.99 0.313291 -0.18
683 778 5hmo (A) 805 903 16.00 0.21 0.07 0.217213 -0.35
691 754 4tql (A) 97 163 19.00 0.31 0.02 0.298208 -0.98
801 1177 3i6u (A) 93 485 42.00 0 1 0.672343 0.2
921 1166 5hu3 (A) 11 260 40.00 0 1 0.624595 -0.44
1040 1118 3vut (B) 230 308 51.00 0.51 0.09 -0.120333 2.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128695)

Species Accession Gene Product
Candida albicans CaO19.4002   kinase involved in DNA repair
Candida albicans CaO19.11485   kinase involved in DNA repair
Candida albicans CaO19.14198   protein kinase
Candida albicans CaO19.6936   protein kinase
Caenorhabditis elegans CELE_T08D2.7   Protein T08D2.7
Caenorhabditis elegans CELE_Y60A3A.12   Protein CHK-2
Dictyostelium discoideum DDB_G0293656   FHA domain-containing protein
Dictyostelium discoideum DDB_G0280321   FHA domain-containing protein
Dictyostelium discoideum DDB_G0281567   FHA domain-containing protein
Dictyostelium discoideum DDB_G0285963   FHA domain-containing protein
Drosophila melanogaster Dmel_CG10895   loki
Echinococcus granulosus EgrG_000772000   serine:threonine protein kinase Chk2
Echinococcus granulosus EgrG_000826300   calcium:calmodulin dependent protein kinase I
Entamoeba histolytica EHI_011510   protein kinase, putative
Entamoeba histolytica EHI_014100   protein kinase, putative
Echinococcus multilocularis EmuJ_000772000   serine:threonine protein kinase Chk2
Echinococcus multilocularis EmuJ_000826300   calcium:calmodulin dependent protein kinase I
Homo sapiens ENSG00000183765   checkpoint kinase 2
Mus musculus ENSMUSG00000029521   checkpoint kinase 2
Saccharomyces cerevisiae YPL153C   Rad53p
Saccharomyces cerevisiae YDL101C   Dun1p
Schistosoma japonicum Sjp_0004230   ko:K06641 serine/threonine-protein kinase Chk2, putative
Schistosoma mansoni Smp_171370   serine/threonine protein kinase
Schmidtea mediterranea mk4.000295.07  
Schmidtea mediterranea mk4.000295.08   Serine/threonine-protein kinase Chk2

Essentiality

Sjp_0004230 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y60A3A.12 Caenorhabditis elegans embryonic lethal wormbase
YPL153C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens checkpoint kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Patiria pectinifera Cdc2 300 aa 23.5% 243 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 24.8% 286 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 23.5% 293 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 21.5% 288 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 23.9% 247 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 28.0% 264 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Sjp_0004230 (Schistosoma japonicum), ko:K06641 serine/threonine-protein kinase Chk2, putative
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