Detailed view for Rv0243

Basic information

TDR Targets ID: 980441
Mycobacterium tuberculosis, Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase)
Source Database / ID:  Tuberculist 

pI: 6.6112 | Length (AA): 440 | MW (Da): 46075 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 439 2iik (B) 29 421 31.00 0 1 1.14393 0.45
11 439 2ib8 (A) 38 425 28.00 0 1 1.1299 0.53
14 439 1ulq (A) 3 398 34.00 0 1 1.25238 0.27
91 126 4wky (A) 182 217 42.00 0.015 0.14 0.323018 1.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131200)

Species Accession Gene Product
Brugia malayi Bm1_45005   Hypothetical 47.9 kDa protein B0303.3 in chromosome III
Caenorhabditis elegans CELE_B0303.3   Protein B0303.3
Drosophila melanogaster Dmel_CG4581   CG4581 gene product from transcript CG4581-RA
Escherichia coli b2342   beta-ketoacyl-CoA thiolase, anaerobic, subunit
Homo sapiens ENSG00000138029   hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit
Leishmania braziliensis LbrM.31.1840   thiolase protein-like protein
Leishmania infantum LinJ.31.1660   3-ketoacyl-CoA thiolase-like protein, putative
Leishmania major LmjF.31.1640   thiolase protein-like protein
Leishmania major LmjF.31.1630   3-ketoacyl-CoA thiolase-like protein, putative
Leishmania mexicana LmxM.30.1630   3-ketoacyl-CoA thiolase-like protein, putative
Leishmania mexicana LmxM.30.1640   thiolase protein-like protein
Loa Loa (eye worm) LOAG_07710   long-chain 3-ketoacyl-CoA thiolase
Mycobacterium leprae ML2564c   PROBABLE ACETYL-CoA ACYLTRANSFERASE FADA2 (3-KETOACYL-CoA THIOLASE) (BETA-KETOTHIOLASE)
Mus musculus ENSMUSG00000059447   hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta sub
Mycobacterium tuberculosis Rv0243   Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase)
Mycobacterium ulcerans MUL_1167   acetyl-CoA acetyltransferase
Onchocerca volvulus OVOC8501   Trifunctional enzyme subunit beta, mitochondrial homolog
Schmidtea mediterranea mk4.001233.00   Trifunctional enzyme subunit beta, mitochondrial
Schmidtea mediterranea mk4.052381.00   Trifunctional enzyme subunit beta, mitochondrial
Trypanosoma cruzi TcCLB.511389.150   thiolase protein-like protein, putative

Essentiality

Rv0243 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b2342 Escherichia coli non-essential goodall
CELE_B0303.3 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0243 (Mycobacterium tuberculosis), Probable acetyl-CoA acyltransferase FadA2 (3-ketoacyl-CoA thiolase) (beta-ketothiolase)
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