Detailed view for Rv3149

Basic information

TDR Targets ID: 980602
Mycobacterium tuberculosis, Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E)

Source Database / ID:  Tuberculist 

pI: 4.5712 | Length (AA): 252 | MW (Da): 27166 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 203 3i9v (2) 3 173 36.00 0 1 1.11464 -0.43
58 240 5xf9 (A) 12 220 24.00 0.4 1 0.89589 0.04
108 192 5abr (A) 5 96 16.00 0 0.91 0.476402 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127830)

Species Accession Gene Product
Arabidopsis thaliana AT4G02580   NADH dehydrogenase (ubiquinone) flavoprotein 2
Brugia malayi Bm1_20470   NADH-ubiquinone oxidoreductase 24 kDa subunit, mitochondrial precursor
Candida albicans CaO19.2091   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUHM, N. crassa and mammalian 2
Candida albicans CaO19.9638   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUHM, N. crassa and mammalian 2
Caenorhabditis elegans CELE_F53F4.10   Protein F53F4.10
Dictyostelium discoideum DDB_G0291173   NADH dehydrogenase
Drosophila melanogaster Dmel_CG5703   CG5703 gene product from transcript CG5703-RA
Escherichia coli b2285   NADH:ubiquinone oxidoreductase, chain E
Echinococcus granulosus EgrG_001114700   NADH dehydrogenase ubiquinone flavoprotein 2
Echinococcus multilocularis EmuJ_001114700   NADH dehydrogenase (ubiquinone) flavoprotein 2
Homo sapiens ENSG00000178127   NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Leishmania braziliensis LbrM.17.0250   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania donovani LdBPK_170320.1   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania infantum LinJ.17.0320   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania major LmjF.17.0270   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Leishmania mexicana LmxM.17.0270   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Loa Loa (eye worm) LOAG_05848   NADH-ubiquinone oxidoreductase 24 kDa subunit
Mus musculus ENSMUSG00000024099   NADH dehydrogenase (ubiquinone) flavoprotein 2
Mycobacterium tuberculosis Rv3149   Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E)
Mycobacterium ulcerans MUL_2463   NADH dehydrogenase subunit E
Oryza sativa 4339271   Os05g0509200
Onchocerca volvulus OVOC1929   Putative NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
Schistosoma japonicum Sjp_0304770   ko:K03943 NADH dehydrogenase (ubiquinone) flavoprotein 2, putative
Schistosoma mansoni Smp_069770   NADH-ubiquinone oxidoreductase
Schmidtea mediterranea mk4.070622.01  
Schmidtea mediterranea mk4.004744.01   Probable NADH dehydrogenase
Trypanosoma brucei gambiense Tbg972.7.7330   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma brucei Tb927.7.6350   NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
Trypanosoma congolense TcIL3000_7_5250   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.511323.50   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trypanosoma cruzi TcCLB.506513.190   NADH-ubiquinone oxidoreductase, mitochondrial, putative
Trichomonas vaginalis TVAG_296220   NADH dehydrogenase 24 kDa subunit, putative
Wolbachia endosymbiont of Brugia malayi Wbm0594   NADH dehydrogenase subunit E

Essentiality

Rv3149 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.6350 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.6350 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.6350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.6350 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F53F4.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_F53F4.10 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3149 (Mycobacterium tuberculosis), Probable NADH dehydrogenase I (chain E) NuoE (NADH-ubiquinone oxidoreductase chain E)
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