Detailed view for Rv3146

Basic information

TDR Targets ID: 981563
Mycobacterium tuberculosis, Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B)

Source Database / ID:  Tuberculist 

pI: 7.7004 | Length (AA): 184 | MW (Da): 20187 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 168 4hea (6) 18 176 63.00 0 1 1.53753 -0.11

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127327)

Species Accession Gene Product
Arabidopsis thaliana AT5G11770   NADH dehydrogenase (ubiquinone) Fe-S protein 7
Brugia malayi Bm1_48645   Probable NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrialprecursor
Candida albicans CaO19.14086   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUKM subunit and mammalian PSST
Candida albicans CaO19.6794   potential mitochondrial NADH-Ubiquinone Oxidoreductase Complex I subunit similar to Y.lipolytica NUKM subunit and mammalian PSST
Caenorhabditis elegans CELE_W10D5.2   Protein NDUF-7
Dictyostelium discoideum DDB_G0285239   NADH dehydrogenase Fe-S protein 7
Drosophila melanogaster Dmel_CG2014   CG2014 gene product from transcript CG2014-RB
Drosophila melanogaster Dmel_CG9172   CG9172 gene product from transcript CG9172-RC
Escherichia coli b2287   NADH:ubiquinone oxidoreductase, chain B
Echinococcus granulosus EgrG_000912900   NADH dehydrogenase ubiquinone iron sulfur
Echinococcus multilocularis EmuJ_000912900   NADH dehydrogenase (ubiquinone) iron sulfur
Homo sapiens ENSG00000115286   NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase)
Leishmania braziliensis LbrM.27.0820   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor
Leishmania donovani LdBPK_270590.1   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Leishmania infantum LinJ.27.0590   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor
Leishmania major LmjF.27.0740   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor
Leishmania mexicana LmxM.27.0740   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor
Loa Loa (eye worm) LOAG_05529   NADH-ubiquinone oxidoreductase Fe-S protein 7
Mus musculus ENSMUSG00000020153   NADH dehydrogenase (ubiquinone) Fe-S protein 7
Mycobacterium tuberculosis Rv3146   Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B)
Mycobacterium ulcerans MUL_2460   NADH dehydrogenase subunit B
Oryza sativa 9267609   Os07g0116900
Oryza sativa 4339427   Os05g0533700
Schistosoma japonicum Sjp_0205730   ko:K03940 NADH dehydrogenase (ubiquinone) Fe-S protein 7, putative
Schistosoma mansoni Smp_092490.2   NADH-plastoquinone oxidoreductase
Schmidtea mediterranea mk4.004322.01   Probable NADH dehydrogenase
Schmidtea mediterranea mk4.016475.01   Probable NADH dehydrogenase
Schmidtea mediterranea mk4.007749.00   Probable NADH dehydrogenase
Schmidtea mediterranea mk4.006988.01   Probable NADH dehydrogenase
Trypanosoma brucei gambiense Tbg972.11.1390   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor, putative
Trypanosoma brucei Tb927.11.1320   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Trypanosoma congolense TcIL3000.11.1260   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Trypanosoma congolense TcIL3000.11.1250   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Trypanosoma cruzi TcCLB.503893.80   NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial precursor
Trypanosoma cruzi TcCLB.508507.59   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Wolbachia endosymbiont of Brugia malayi Wbm0242   NADH dehydrogenase subunit B

Essentiality

Rv3146 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.47.0017 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0017 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0017 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0017 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2287 Escherichia coli non-essential goodall
CELE_W10D5.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_W10D5.2 Caenorhabditis elegans larval arrest wormbase
CELE_W10D5.2 Caenorhabditis elegans slow growth wormbase
CELE_W10D5.2 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv3146 (Mycobacterium tuberculosis), Probable NADH dehydrogenase I (chain B) NuoB (NADH-ubiquinone oxidoreductase chain B)
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