Detailed view for PF3D7_1343600

Basic information

TDR Targets ID: 129
Plasmodium falciparum, UDP-N-acetylglucosamine pyrophosphorylase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5637 | Length (AA): 593 | MW (Da): 69984 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01704   UTP--glucose-1-phosphate uridylyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0070569   GO:uridylyltransferase activity  

GO:0006047   UDP-N-acetylglucosamine metabolic process  
GO:0003977   UDP-N-acetylglucosamine diphosphorylase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
138 591 1z90 (A) 24 420 16.00 0 0.95 0.8 0.29
1 590 1jv1 (A) 3 495 29.00 0 1 0.930141 1.02
2 587 2yqc (A) 7 486 26.00 0 1 0.818696 0.83
36 216 4u5a (C) 47 239 16.00 0.53 0.14 0.398428 -0.22

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, gametocyte, early trophozoite, late trophozoite, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, Oocyst, Ring, Sporozoite, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_127359)

Species Accession Gene Product
Arabidopsis thaliana AT1G31070   N-acetylglucosamine-1-phosphate uridylyltransferase 1
Arabidopsis thaliana AT2G35020   putative UDP-N-acetylglucosamine pyrophosphorylase
Babesia bovis BBOV_I003580   UDP-N-acetylglucosamine pyrophosphorylase, putative
Brugia malayi Bm1_39285   UTP--glucose-1-phosphate uridylyltransferase family protein
Candida albicans CaO19.11741   UDP-N-acetylglucosamine pyrophosphorylase
Candida albicans CaO19.4265   UDP-N-acetylglucosamine pyrophosphorylase
Caenorhabditis elegans CELE_C36A4.4   Protein C36A4.4
Caenorhabditis elegans CELE_K06B9.2   Protein K06B9.2
Cryptosporidium hominis Chro.40100   UDP-N-acetylglucosamine pyrophosphorylase; Qri1p
Cryptosporidium parvum cgd4_810   UDP-N-acetylglucosamine pyrophosphorylase
Chlamydia trachomatis CT_715   UDP-glucose pyrophosphorylase
Dictyostelium discoideum DDB_G0290055   UDP-N-acetylgalactosamine pyrophosphorylase
Drosophila melanogaster Dmel_CG9535   mummy
Echinococcus granulosus EgrG_000774100   UDP N acetylhexosamine pyrophosphorylase
Entamoeba histolytica EHI_021200   UDP-N-acetylglucosamine pyrophosphorylase, putative
Entamoeba histolytica EHI_039830   UDP-N-acetylglucosamine pyrophosphorylase, putative
Echinococcus multilocularis EmuJ_000774100   UDP N acetylhexosamine pyrophosphorylase
Giardia lamblia GL50803_16217   UDP-N-acetylglucosamine pyrophosphorylase
Homo sapiens ENSG00000117143   UDP-N-acetylglucosamine pyrophosphorylase 1
Homo sapiens ENSG00000197355   UDP-N-acetylglucosamine pyrophosphorylase 1 like 1
Leishmania braziliensis LbrM.33.2790   UDP-N-acetylglucosamine pyrophosphorylase, putative
Leishmania donovani LdBPK_332650.1   UDP-N-acetylglucosamine pyrophosphorylase, putative
Leishmania infantum LinJ.33.2650   UDP-N-acetylglucosamine pyrophosphorylase, putative
Leishmania major LmjF.33.2520   UDP-N-acetylglucosamine pyrophosphorylase, putative
Leishmania mexicana LmxM.32.2520   UDP-N-acetylglucosamine pyrophosphorylase, putative
Loa Loa (eye worm) LOAG_00239   UTP-glucose-1-phosphate uridylyltransferase
Mus musculus 102642017   UDP-N-acetylhexosamine pyrophosphorylase-like
Mus musculus 227620   UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
Mus musculus ENSMUSG00000026670   UDP-N-acetylglucosamine pyrophosphorylase 1
Neospora caninum NCLIV_040400   hypothetical protein
Oryza sativa 4344934   Os08g0206900
Oryza sativa 4336977   Os04g0613700
Plasmodium berghei PBANKA_1356600   UDP-N-acetylglucosamine pyrophosphorylase, putative
Plasmodium falciparum PF3D7_1343600   UDP-N-acetylglucosamine pyrophosphorylase, putative
Plasmodium knowlesi PKNH_1257800   UDP-N-acetylglucosamine pyrophosphorylase, putative
Plasmodium vivax PVX_083075   UDP-N-acteylglucosamine pyrophosphorylase 1, putative
Plasmodium yoelii PY02947   UDP-n-acetylglucosamine pyrophosphorylase
Saccharomyces cerevisiae YDL103C   UDP-N-acetylglucosamine diphosphorylase
Schistosoma japonicum Sjp_0039630   ko:K00972 UDP-N-acetylglucosamine pyrophosphorylase [EC2.7.7.23], putative
Schistosoma mansoni Smp_043350   UDP-n-acetylglucosamine pyrophosphorylase
Schmidtea mediterranea mk4.022219.01  
Schmidtea mediterranea mk4.038701.00   UDP-n-acetylglucosamine pyrophosphorylase
Schmidtea mediterranea mk4.011751.03  
Schmidtea mediterranea mk4.020664.00   Mummy
Trypanosoma brucei gambiense Tbg972.11.2760   UDP-N-acetylglucosamine pyrophosphorylase, putative
Trypanosoma brucei Tb927.11.2520   UDP-n-acetylglucosamine pyrophosphorylase
Trypanosoma cruzi TcCLB.504153.180   UDP-N-acetylglucosamine pyrophosphorylase, putative
Toxoplasma gondii TGME49_264780   UTP-glucose-1-phosphate uridylyltransferase subfamily protein
Theileria parva TP01_0489   UDP-N-acetylglucosamine pyrophosphorylase, putative
Trichomonas vaginalis TVAG_133060   UDP- glucose pyrophosphorylase, putative

Essentiality

PF3D7_1343600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.0120 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.0120 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0120 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C36A4.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C36A4.4 Caenorhabditis elegans sterile wormbase
CELE_K06B9.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_K06B9.2 Caenorhabditis elegans sterile wormbase
YDL103C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1356600 Plasmodium berghei Slow plasmo
TGME49_264780 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1343600 (Plasmodium falciparum), UDP-N-acetylglucosamine pyrophosphorylase, putative
Title for this comment
Comment