Detailed view for LmjF.15.1250

Basic information

TDR Targets ID: 27761
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6405 | Length (AA): 171 | MW (Da): 19442 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08694   Ubiquitin-fold modifier-conjugating enzyme 1

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 159 1ywz (A) 3 160 61.00 0 1 1.68 -0.69
4 160 3kpa (A) 4 160 99.00 0 1 2.18333 -1.94

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3KPA:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130588)

Species Accession Gene Product
Arabidopsis thaliana AT1G27530   ubiquitin-fold modifier-conjugating enzyme 1
Babesia bovis BBOV_IV006180   Ufm1-conjugating enzyme 1, putative
Brugia malayi Bm1_46190   Protein CGI-126
Caenorhabditis elegans CELE_C40H1.6   Protein C40H1.6
Cryptosporidium parvum cgd8_3630   conserved hypothetical protein
Dictyostelium discoideum DDB_G0289037   ubiquitin-like post-translational modifier
Drosophila melanogaster Dmel_CG8386   CG8386 gene product from transcript CG8386-RA
Echinococcus granulosus EgrG_001080700   ubiquitin fold modifier conjugating enzyme
Echinococcus multilocularis EmuJ_001080700   ubiquitin fold modifier conjugating enzyme
Homo sapiens ENSG00000143222   ubiquitin-fold modifier conjugating enzyme 1
Leishmania braziliensis LbrM.15.1190   hypothetical protein, conserved
Leishmania donovani LdBPK_151270.1   E2-like ubiquitin-conjugation enzyme, putative
Leishmania infantum LinJ.15.1270   hypothetical protein, conserved
Leishmania major LmjF.15.1250   hypothetical protein, conserved
Leishmania mexicana LmxM.15.1250   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03349   ubiquitin-fold modifier-conjugating enzyme 1
Mus musculus ENSMUSG00000062963   ubiquitin-fold modifier conjugating enzyme 1
Neospora caninum NCLIV_013570   hypothetical protein, conserved
Oryza sativa 4348269   Os10g0205200
Schistosoma japonicum Sjp_0068070   Ufm1-conjugating enzyme 1, putative
Schistosoma mansoni Smp_070770   hypothetical protein
Schmidtea mediterranea mk4.000015.09   Ubiquitin-fold modifier-conjugating enzyme 1
Trypanosoma brucei gambiense Tbg972.9.2990   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.5650   E2-like ubiquitin-conjugation enzyme, putative
Trypanosoma congolense TcIL3000_9_1890   E2-like ubiquitin-conjugation enzyme, putative
Trypanosoma cruzi TcCLB.506445.100   E2-like ubiquitin-conjugation enzyme, putative
Toxoplasma gondii TGME49_213830   Ufm1-conjugating enzyme 1, putative
Theileria parva TP01_0425   hypothetical protein, conserved

Essentiality

LmjF.15.1250 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.4150 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.160.4150 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.4150 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.4150 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_213830 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Lmaj001866AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Lmaj001866; Recombinant protein: full-length; Source: L major; hypothetical protein, conserved ;

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.15.1250 (Leishmania major), hypothetical protein, conserved
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