Detailed view for PF3D7_0201800

Basic information

TDR Targets ID: 3290
Plasmodium falciparum, knob associated heat shock protein 40
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.5764 | Length (AA): 421 | MW (Da): 48283 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain
PF01556   DnaJ C terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
49 159 2ctq (A) 3 110 25.00 0 1 0.53 0.14
63 136 2ctq (A) 16 86 35.00 0.00000000077 1 0.71 -1.23
67 159 1fpo (A) 1 97 16.00 0.0000000099 0.95 0.46 -0.29
171 382 1nlt (A) 128 337 28.00 0 1 0.73 1.24
305 397 1xao (A) 259 354 37.00 0 1 0.66 -0.95
87 155 2qsa (A) 35 104 41.00 0.0000017 1 0.854895 -2
88 418 4j80 (A) 6 268 28.00 0.000071 1 0.772223 1.22
88 156 2cug (A) 18 82 60.00 0 1 0.932895 -1.38
245 416 2q2g (A) 8 179 54.00 0 1 1.08755 -0.48
88 418 4j80 (A) 6 268 28.00 0.00016 1 0.794123 0.98
245 416 2q2g (A) 8 179 55.00 0 1 1.09095 -0.41

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, merozoite, sporozoite, early ring, early trophozoite, late ring, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 32 hs, late schizont, late trophozoite. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Oocyst, Female Gametocyte, Male Gametocyte. Zanghi G Lasonder E
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_126773)

Species Accession Gene Product
Arabidopsis thaliana AT3G08910   DnaJ domain-containing protein
Arabidopsis thaliana AT2G20560   DNAJ heat shock family protein
Arabidopsis thaliana AT5G25530   putative DNAJ heat shock protein
Arabidopsis thaliana AT1G10350   putative DNAJ heat-shock protein
Arabidopsis thaliana AT1G59725   putative DNAJ heat shock protein
Babesia bovis BBOV_II006560   protein with DnaJ domain, DNJ1/SIS1 family
Brugia malayi Bm1_16640   DnaJ homolog subfamily B member 4
Candida albicans CaO19.3861   DnaJ-like protein similar to S. cerevisiae SIS1 (YNL007C) Sit4 suppressor
Candida albicans CaO19.11342   DnaJ-like protein similar to S. cerevisiae SIS1 (YNL007C) HSP40 family chaperone
Caenorhabditis elegans CELE_F54D5.8   Protein DNJ-13
Cryptosporidium hominis Chro.20195   heat shock 40 kDa protein
Cryptosporidium parvum cgd2_1800   heat shock 40 kDa protein, putative
Drosophila melanogaster Dmel_CG10578   DnaJ-like-1
Drosophila melanogaster Dmel_CG5001   CG5001 gene product from transcript CG5001-RB
Echinococcus granulosus EgrG_000614200   dnaJ subfamily B
Echinococcus granulosus EgrG_000658700   dnaJ subfamily B 3
Echinococcus granulosus EgrG_000657800   dnaJ subfamily B 3
Entamoeba histolytica EHI_187000   DnaJ family protein
Entamoeba histolytica EHI_183280   DnaJ family protein
Echinococcus multilocularis EmuJ_000657800  
Echinococcus multilocularis EmuJ_000658700   dnaJ subfamily B 3
Echinococcus multilocularis EmuJ_000614200   dnaJ subfamily B
Giardia lamblia GL50803_17483   Chaperone protein dnaJ
Homo sapiens ENSG00000187726   DnaJ (Hsp40) homolog, subfamily B, member 13
Homo sapiens ENSG00000137094   DnaJ (Hsp40) homolog, subfamily B, member 5
Homo sapiens 11080   DnaJ (Hsp40) homolog, subfamily B, member 4
Homo sapiens ENSG00000132002   DnaJ (Hsp40) homolog, subfamily B, member 1
Leishmania braziliensis LbrM.35.6590   chaperone protein DNAj, putative
Leishmania donovani LdBPK_366530.1   chaperone protein DnaJ, putative
Leishmania infantum LinJ.36.6530   chaperone protein DNAj, putative
Leishmania major LmjF.36.6270   chaperone protein DNAj, putative
Leishmania mexicana LmxM.36.6270   chaperone protein DNAj, putative
Loa Loa (eye worm) LOAG_01435   hypothetical protein
Loa Loa (eye worm) LOAG_08423   dnaJ-class molecular chaperone
Mus musculus ENSMUSG00000005483   DnaJ (Hsp40) homolog, subfamily B, member 1
Mus musculus ENSMUSG00000036052   DnaJ (Hsp40) homolog, subfamily B, member 5
Mus musculus ENSMUSG00000030708   DnaJ (Hsp40) related, subfamily B, member 13
Mus musculus ENSMUSG00000028035   DnaJ (Hsp40) homolog, subfamily B, member 4
Neospora caninum NCLIV_039960   hypothetical protein
Oryza sativa 4329116   Os02g0306900
Oryza sativa 4326364   Os01g0239100
Oryza sativa 4339609   Os05g0562300
Oryza sativa 4344726   Os08g0161900
Onchocerca volvulus OVOC11163  
Plasmodium berghei PBANKA_0310000   protein SIS1, putative
Plasmodium falciparum PF3D7_0213100   protein SIS1
Plasmodium falciparum PF3D7_0501100   heat shock protein 40, type II
Plasmodium falciparum PF3D7_0201800   knob associated heat shock protein 40
Plasmodium knowlesi PKNH_1246700   DNAJ like protein, putative
Plasmodium knowlesi PKNH_0407900   protein SIS1, putative
Plasmodium vivax PVX_002875   protein SIS1, putative
Plasmodium vivax PVX_118690   40 kDa heat shock protein, putative
Plasmodium yoelii PY02986   DnaJ C terminal region, putative
Saccharomyces cerevisiae YNL007C   Sis1p
Schistosoma japonicum Sjp_0050730   DnaJ homolog subfamily B member 5, putative
Schistosoma japonicum Sjp_0094860   ko:K09510 DnaJ homolog, subfamily B, member 4, putative
Schistosoma japonicum Sjp_0209050   ko:K09519 DnaJ homolog, subfamily B, member 13, putative
Schistosoma mansoni Smp_020920.1   DNAj homolog subfamily B member 4
Schistosoma mansoni Smp_020920.2   DNAj homolog subfamily B member 4
Schistosoma mansoni Smp_104730   DNAj homolog subfamily B member 4
Schmidtea mediterranea mk4.000171.01   Hsp40
Schmidtea mediterranea mk4.002178.02   DnaJ homolog subfamily B member 13
Schmidtea mediterranea mk4.001362.08   DnaJ homolog subfamily B member 13
Schmidtea mediterranea mk4.001362.07  
Trypanosoma brucei gambiense Tbg972.10.10330   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.10.8540   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_10_7330   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.506355.50   chaperone DNAJ protein, putative
Trypanosoma cruzi TcCLB.511043.30   DnaJ chaperone protein, putative
Toxoplasma gondii TGME49_265310   heat shock protein 40, putative
Theileria parva TP02_0625   dnaJ protein, putative
Trichomonas vaginalis TVAG_347420   DNAj/HSP40, putative
Trichomonas vaginalis TVAG_317210   DNAj/HSP40, putative

Essentiality

PF3D7_0201800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.8540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.8540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.8540 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.8540 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F54D5.8 Caenorhabditis elegans sterile wormbase
YNL007C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0310000 Plasmodium berghei Dispensable plasmo
TGME49_265310 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_0201800 (Plasmodium falciparum), knob associated heat shock protein 40
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