Detailed view for Rv0013

Basic information

TDR Targets ID: 9991
Mycobacterium tuberculosis, Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
Source Database / ID:  Tuberculist 

pI: 6.491 | Length (AA): 232 | MW (Da): 24627 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00117   Glutamine amidotransferase class-I

Gene Ontology

Mouse over links to read term descriptions.
GO:0004049   anthranilate synthase activity  
GO:0003824   catalytic activity  
GO:0009058   biosynthetic process  
GO:0008152   metabolic process  
GO:0006541   glutamine metabolic process  

Metabolic Pathways

Folate biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 189 1qdl (B) 3 192 43.00 0 1 1.37004 -0.98
2 232 1gpm (A) 9 241 23.00 0 1 1.28829 -0.18
19 61 3aam (A) 72 116 44.00 0.47 0.41 0.556545 0.06
2 189 1qdl (B) 3 192 43.00 0 1 1.37004 -0.98
2 232 1gpm (A) 9 241 23.00 0 1 1.28829 -0.18
19 61 3aam (A) 72 116 44.00 0.47 0.41 0.556545 0.06
2 189 1qdl (B) 3 192 43.00 0 1 1.37004 -0.98
2 232 1gpm (A) 9 241 23.00 0 1 1.28829 -0.18
19 61 3aam (A) 72 116 44.00 0.47 0.41 0.556545 0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_128663)

Species Accession Gene Product
Arabidopsis thaliana AT1G24807   putative anthranilate synthase beta subunit
Arabidopsis thaliana AT1G25083   putative anthranilate synthase beta subunit
Arabidopsis thaliana AT1G25155   Glutamine amidotransferase type 1 family protein
Arabidopsis thaliana AT5G57890   glutamine amidotransferase type 1 family protein
Arabidopsis thaliana AT1G24909   putative anthranilate synthase beta subunit
Arabidopsis thaliana AT1G25220   anthranilate synthase beta subunit 1
Candida albicans CaO19.5243   likely bifunctional tryptophan biosynthesis enzyme similar to S. cerevisiae TRP3 (YKL211C) indole-3-glycerol phosphate synthase
Candida albicans CaO19.12708   likely bifunctional tryptophan biosynthesis enzyme similar to S. cerevisiae TRP3 (YKL211C) indole-3-glycerol phosphate synthase
Escherichia coli b3360   aminodeoxychorismate synthase, subunit II
Mycobacterium leprae ML0015   Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
Mycobacterium tuberculosis Rv0013   Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
Mycobacterium ulcerans MUL_0017   para-aminobenzoate synthase component II
Neospora caninum NCLIV_022000   para-aminobenzoate synthase, putative
Oryza sativa 4336076   Os04g0463500
Oryza sativa 4333918   Os03g0718000
Saccharomyces cerevisiae YKL211C   bifunctional anthranilate synthase/indole-3-glycerol-phosphate synthase

Essentiality

Rv0013 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3360 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0013 (Mycobacterium tuberculosis), Possible anthranilate synthase component II TrpG (glutamine amidotransferase)
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