Detailed view for Rv0346c

Basic information

TDR Targets ID: 5665
Mycobacterium tuberculosis, Possible L-asparagine permease AnsP2 (L-asparagine transport protein)
Source Database / ID:  Tuberculist 

pI: 9.5502 | Length (AA): 487 | MW (Da): 52195 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 12

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00324   Amino acid permease

Gene Ontology

Mouse over links to read term descriptions.
GO:0022857   transmembrane transporter activity  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0015171   amino acid transmembrane transporter activity  
GO:0006865   amino acid transport  
GO:0006810   transport  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 431 5oqt (A) 8 429 25.00 0 0.95 1.1398 -0.43
19 463 3gia (A) 5 425 20.00 0 0.57 1.10326 -0.45
29 425 5j4i (A) 17 396 24.00 0 1 1.0471 -0.01
138 286 5c6n (A) 167 305 31.00 0.58 0.23 0.0678548 3.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127087)

Species Accession Gene Product
Candida albicans CaO19.97   one of four genes similar to S. cerevisiae CAN1 (YEL063C) arginine permease
Candida albicans CaO19.3195   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.2337   likely amino acid permease similar to several bacterial lysine permeases and also to S. cerevisiae LYP1 (YNL268W), ALP1 (YNL270C
Candida albicans CaO19.9873   likely amino acid permease similar to several bacterial lysine permeases and also to S. cerevisiae LYP1 (YNL268W), ALP1 (YNL270C
Candida albicans CaO19.10706   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.111   one of four genes similar to S. cerevisiae CAN1 (YEL063C) arginine permease
Candida albicans CaO19.11123   one of four genes similar to S. cerevisiae CAN1 (YEL063C) arginine permease
Candida albicans CaO19.6659   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.1799   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.3641   one of four genes similar to S. cerevisiae CAN1 (YEL063C) arginine permease
Candida albicans CaO19.6993   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.11936   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.4456   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.7758   one of four genes similar to S. cerevisiae CAN1 (YEL063C) arginine permease
Candida albicans CaO19.11780   N-acetylglucosamine-inducible general amino acid permease that co-ordinates external nitrogen source response and morphogenesis
Candida albicans CaO19.9365   one of seven likely amino acid permease genes similar to S. cerevisiae GAP1 (YKR039W) general amino acid permease
Candida albicans CaO19.4304   N-acetylglucosamine-inducible general amino acid permease that co-ordinates external nitrogen source response and morphogenesis
Escherichia coli b2156   lysine transporter
Escherichia coli b1453   L-asparagine transporter
Mycobacterium leprae ML1305c   POSSIBLE L-ASPARAGINE PERMEASE ANSP2 (L-ASPARAGINE TRANSPORT PROTEIN)
Mycobacterium leprae ML1304c   POSSIBLE L-ASPARAGINE PERMEASE ANSP1 (L-ASPARAGINE TRANSPORT PROTEIN)
Mycobacterium tuberculosis Rv0346c   Possible L-asparagine permease AnsP2 (L-asparagine transport protein)
Mycobacterium tuberculosis Rv2127   L-asparagine permease AnsP1
Mycobacterium ulcerans MUL_0383   L-asparagine permease AnsP1
Saccharomyces cerevisiae YEL063C   Can1p
Saccharomyces cerevisiae YNL270C   Alp1p
Saccharomyces cerevisiae YNL268W   Lyp1p
Saccharomyces cerevisiae YKR039W   amino acid permease GAP1
Trichomonas vaginalis TVAG_021750   amino acid permease, putative
Trichomonas vaginalis TVAG_480660   aromatic amino acid transporter, putative
Trichomonas vaginalis TVAG_056200   lysine-specific permease, putative
Trichomonas vaginalis TVAG_084860   gaba permease, putative

Essentiality

Rv0346c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu349 this record Mycobacterium tuberculosis non-essential nmpdr
b1453 Escherichia coli non-essential goodall
b2156 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv0346c (Mycobacterium tuberculosis), Possible L-asparagine permease AnsP2 (L-asparagine transport protein)
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