Detailed view for Rv0350

Basic information

TDR Targets ID: 5883
Mycobacterium tuberculosis, Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70)
Source Database / ID:  Tuberculist 

pI: 4.5884 | Length (AA): 625 | MW (Da): 66831 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00012   Hsp70 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0005524   ATP binding  
GO:0006457   protein folding  

Metabolic Pathways

RNA degradation (KEGG)
Tuberculosis (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 357 4rtf (D) 3 357 99.99 0 1 1.7837 -1.34
4 584 5tky (A) 9 610 44.00 0 1 1.4193 -0.58
360 614 5gjj (A) 4 257 48.00 0 1 0.7947 1.05
367 514 3dob (A) 396 544 63.00 0 1 1.1045 -1.66
485 624 5cwi (A) 2 141 24.00 0 0.48 0.5667 -0.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4RTF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_126744)

Species Accession Gene Product
Arabidopsis thaliana AT4G37910   heat shock protein 70-1
Arabidopsis thaliana AT5G49910   heat shock protein 70-2
Arabidopsis thaliana AT5G09590   mitochondrial HSO70 2
Arabidopsis thaliana AT4G24280   chloroplast heat shock protein 70-1
Babesia bovis BBOV_IV003490   heat shock protein 70
Brugia malayi Bm1_51555   Heat shock 70 kDa protein F, mitochondrial precursor, putative
Candida albicans CaO19.7179   heat shock protein
Candida albicans CaO19_7179   hypothetical protein
Candida albicans CaO19.9452   Mitochondrial import protein (DnaK)
Candida albicans CaO19.1896   Mitochondrial import protein (DnaK)
Caenorhabditis elegans CELE_C37H5.8   Protein HSP-6
Cryptosporidium hominis Chro.30389   dnaK-type molecular chaperone hsp70, organellar
Cryptosporidium parvum cgd3_3440   heat shock protein HSP70, mitochondrial
Chlamydia trachomatis CT_396   chaperone protein DnaK
Dictyostelium discoideum DDB_G0293298   hypothetical protein
Drosophila melanogaster Dmel_CG8542   Heat shock protein cognate 5
Escherichia coli b0014   chaperone Hsp70, with co-chaperone DnaJ
Echinococcus granulosus EgrG_000909900   stress 70 protein mitochondrial
Echinococcus granulosus EgrG_001065400   heat shock protein 70
Entamoeba histolytica EHI_013760   heat shock protein 70, mitochondrial, putative
Entamoeba histolytica EHI_101120   heat shock protein 70, mitochondrial, putative
Entamoeba histolytica EHI_071800   heat shock protein70, mitochondrial, putative
Entamoeba histolytica EHI_007150   mitochondrial-type heat shock protein 70
Entamoeba histolytica EHI_127700   heat shock protein 70, mitochondrial, putative
Echinococcus multilocularis EmuJ_001065400   heat shock protein 70
Echinococcus multilocularis EmuJ_000909900   heat shock 70 kDa protein, mitochondrial precursor, putative
Homo sapiens ENSG00000113013   heat shock 70kDa protein 9 (mortalin)
Leishmania braziliensis LbrM.30.2420   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania braziliensis LbrM.30.2440   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania infantum LinJ.30.2470   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania infantum LinJ.30.2480   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania infantum LinJ.30.2540   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania infantum LinJ.30.2530   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania major LmjF.30.2550   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania major LmjF.30.2490   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania major LmjF.30.2470   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania major LmjF.30.2460   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania major LmjF.30.2480   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania mexicana LmxM.29.2490   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania mexicana LmxM.29.2460   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania mexicana LmxM.29.2550   heat shock 70-related protein 1, mitochondrial precursor, putative
Leishmania mexicana LmxM.29.2470   heat shock 70-related protein 1, mitochondrial precursor, putative
Loa Loa (eye worm) LOAG_00057   hypothetical protein
Loa Loa (eye worm) LOAG_00058   hypothetical protein
Mycobacterium leprae ML2496c   PROBABLE CHAPERONE PROTEIN DNAK (HEAT SHOCK PROTEIN 70) (HEAT SHOCK 70 KDA PROTEIN) (HSP70)
Mus musculus ENSMUSG00000024359   heat shock protein 9
Mycobacterium tuberculosis Rv0350   Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70)
Mycobacterium ulcerans MUL_3422   chaperone protein DnaK1
Mycobacterium ulcerans MUL_0593   molecular chaperone DnaK
Neospora caninum NCLIV_066840   hypothetical protein
Oryza sativa 4331379   Os03g0113700
Oryza sativa 4338354   Os05g0303000
Oryza sativa 4351875   Os12g0244100
Oryza sativa 9270165   Os09g0491772
Oryza sativa 4330888   Os02g0774300
Onchocerca volvulus OVOC6877   Heat shock 70 kDa protein F, mitochondrial homolog
Onchocerca volvulus OVOC12759  
Plasmodium berghei PBANKA_0914400   heat shock protein 70, putative
Plasmodium falciparum PF3D7_1134000   heat shock protein 70
Plasmodium knowlesi PKNH_0932200   heat shock protein 70, putative
Plasmodium vivax PVX_092310   heat shock protein 70, putative
Plasmodium yoelii PY06981   heat shock protein hsp70 homologue Pfhsp70-3
Saccharomyces cerevisiae YEL030W   Hsp70 family ATPase ECM10
Saccharomyces cerevisiae YJR045C   Hsp70 family ATPase SSC1
Saccharomyces cerevisiae YLR369W   Hsp70 family ATPase SSQ1
Schistosoma japonicum Sjp_0026230   ko:K04043 molecular chaperone DnaK, putative
Schistosoma mansoni Smp_106130.2   heat shock protein 70
Schistosoma mansoni Smp_106130.1   heat shock protein 70
Trypanosoma brucei gambiense Tbg972.6.3520   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma brucei gambiense Tbg972.6.3510   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma brucei gambiense Tbg972.6.3580   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma brucei Tb927.6.3800   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma brucei Tb927.6.3740   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma brucei Tb927.6.3750   heat shock 70 kDa protein, mitochondrial precursor, putative
Trypanosoma congolense TcIL3000_0_08300   heat shock 70 kDa protein, putative
Trypanosoma congolense TcIL3000_0_27070   heat shock 70 kDa protein, putative
Trypanosoma cruzi TcCLB.507029.30   heat shock 70 kDa protein, mitochondrial precursor, putative
Toxoplasma gondii TGME49_251780   heat shock protein
Treponema pallidum TP0216   molecular chaperone DnaK
Theileria parva TP02_0753   heat shock protein 70, putative
Trichomonas vaginalis TVAG_237140   heat shock protein, putative
Trichomonas vaginalis TVAG_340390   heat shock protein 70 (HSP70)-4, putative
Trichomonas vaginalis TVAG_433130   heat shock protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0495   molecular chaperone DnaK

Essentiality

Rv0350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu353 this record Mycobacterium tuberculosis essential nmpdr
Tb927.6.3740 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.3740 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.3740 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.3740 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C37H5.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_C37H5.8 Caenorhabditis elegans larval arrest wormbase
CELE_C37H5.8 Caenorhabditis elegans slow growth wormbase
CELE_C37H5.8 Caenorhabditis elegans sterile wormbase
YJR045C Saccharomyces cerevisiae inviable yeastgenome
TGME49_251780 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Escherichia coli chaperone Hsp70, with co-chaperone DnaJ Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

232 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Rv0350 (Mycobacterium tuberculosis), Probable chaperone protein DnaK (heat shock protein 70) (heat shock 70 kDa protein) (HSP70)
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