Detailed view for Rv0500

Basic information

TDR Targets ID: 6929
Mycobacterium tuberculosis, PROBABLE PYRROLINE-5-CARBOXYLATE REDUCTASE PROC (P5CR) (P5C REDUCTASE)

Source Database / ID: Tuberculist / Rv0500 

pI: 4.4181 | Length (AA): 295 | MW (Da): 30172 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: ND

Pfam domains

PF03807   NADP oxidoreductase coenzyme F420-dependent
PF14748   Pyrroline-5-carboxylate reductase dimerisation

Gene Ontology

Mouse over links to read term descriptions.
GO:0004735   pyrroline-5-carboxylate reductase activity  
GO:0003824   catalytic activity  
GO:0005488   binding  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006561   proline biosynthetic process  

Network

Metabolic Pathways

Structural information

Modbase 3D models: 0

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Shows this level of upregulation in
This gene ranks in the lower 0-20% group of genes. Dormant phase, Dormant phase.
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_126801)
Species Accession Gene Product
Arabidopsis thaliana AT5G14800   Pyrroline-5-carboxylate reductase
Brugia malayi Bm1_38015   pyrroline-5-carboxylate reductase family protein
Candida albicans CaO19.5650   Delta-1-pyrroline-5-carboxylate reductase
Candida albicans CaO19.13095   Delta-1-pyrroline-5-carboxylate reductase
Caenorhabditis elegans CELE_M153.1   Protein M153.1
Cryptosporidium hominis Chro.60426   pyrroline-5-carboxylate reductase
Cryptosporidium parvum cgd6_3720   pyrroline-5-carboxylate reductase
Dictyostelium discoideum DDB_G0269410   PCA reductase
Dictyostelium discoideum DDB_G0288671   PCA reductase
Drosophila melanogaster Dmel_CG6009   Pyrroline 5-carboyxlate reductase
Drosophila melanogaster Dmel_CG5840   Pyrroline-5-carboxylate reductase-like 2
Escherichia coli b0386   pyrroline-5-carboxylate reductase, NAD(P)-binding
Echinococcus granulosus EgrG_000233100   pyrroline 5 carboxylate reductase
Echinococcus multilocularis EmuJ_000233100   pyrroline 5 carboxylate reductase
Homo sapiens ENSG00000104524   pyrroline-5-carboxylate reductase-like
Homo sapiens ENSG00000183010   pyrroline-5-carboxylate reductase 1
Leishmania braziliensis LbrM.13.1540   pyrroline-5-carboxylate reductase
Leishmania donovani LdBPK_131420.1   pyrroline-5-carboxylate reductase
Leishmania infantum LinJ.13.1420   pyrroline-5-carboxylate reductase
Leishmania major LmjF.13.1680   pyrroline-5-carboxylate reductase
Leishmania mexicana LmxM.13.1680   pyrroline-5-carboxylate reductase
Loa Loa (eye worm) LOAG_07911   hypothetical protein
Mycobacterium leprae ML2430   Pyrroline-5-carboxylate reductase
Mus musculus ENSMUSG00000022571   pyrroline-5-carboxylate reductase-like
Mus musculus ENSMUSG00000025140   pyrroline-5-carboxylate reductase 1
Mus musculus ENSMUSG00000026520   pyrroline-5-carboxylate reductase family, member 2
Mycobacterium tuberculosis Rv0500 this record   PROBABLE PYRROLINE-5-CARBOXYLATE REDUCTASE PROC (P5CR) (P5C REDUCTASE)
Mycobacterium ulcerans MUL_4570   pyrroline-5-carboxylate reductase
Neospora caninum NCLIV_050390   Pyrroline-5-carboxylate reductase (EC 1.5.1.2), related
Oryza sativa 4325755   Os01g0948400
Plasmodium berghei PBANKA_1134200   pyrroline-5-carboxylate reductase, putative
Plasmodium falciparum PF3D7_1357900   pyrroline-5-carboxylate reductase
Plasmodium knowlesi PKNH_1113600   pyrroline-5-carboxylate reductase, putative
Plasmodium vivax PVX_114870   pyrroline carboxylate reductase, putative
Plasmodium yoelii PY02938   pyrroline-5-carboxylate reductase
Saccharomyces cerevisiae YER023W   pyrroline-5-carboxylate reductase
Schistosoma japonicum Sjp_0303670   ko:K00286 pyrroline-5-carboxylate reductase [EC1.5.1.2], putative
Schistosoma mansoni Smp_049390   pyrroline-5-carboxylate reductase
Schmidtea mediterranea mk4.015283.01  
Schmidtea mediterranea mk4.023354.00   Pyrroline-5-carboxylate reductase
Trypanosoma brucei gambiense Tbg972.7.2720   pyrroline-5-carboxylate reductase, putative
Trypanosoma brucei Tb927.7.2440   pyrroline-5-carboxylate reductase, putative
Trypanosoma congolense TcIL3000_7_1750   pyrroline-5-carboxylate reductase, putative
Trypanosoma cruzi TcCLB.509207.90   pyrroline-5-carboxylate reductase, putative
Trypanosoma cruzi TcCLB.506857.20   pyrroline-5-carboxylate reductase, putative
Toxoplasma gondii TGME49_236070   pyrroline-5-carboxylate reductase
Treponema pallidum TP0797   1-pyrroline-5-carboxylate reductase (proC)
Trichomonas vaginalis TVAG_423640   pyrroline-5-carboxylate reductase, putative
Trichomonas vaginalis TVAG_063510   pyrroline-5-carboxylate reductase, putative

Essentiality

Rv0500 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu506 this record Mycobacterium tuberculosis essential nmpdr
Tb927.7.2440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.2440 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0386 Escherichia coli non-essential goodall
YER023W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1134200 Plasmodium berghei Dispensable plasmo

Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Curated from literature

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Arabidopsis thaliana Pyrroline-5-carboxylate reductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Rv0500 (Mycobacterium tuberculosis), PROBABLE PYRROLINE-5-CARBOXYLATE REDUCTASE PROC (P5CR) (P5C REDUCTASE)
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TDR Targets Development Release v6, Revision: 1335 (10.Aug.2018)
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