Detailed view for Rv0619

Basic information

TDR Targets ID: 6121
Mycobacterium tuberculosis, Probable galactose-1-phosphate uridylyltransferase GalTb [second part]
Source Database / ID:  Tuberculist 

pI: 9.175 | Length (AA): 181 | MW (Da): 20745 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02744   Galactose-1-phosphate uridyl transferase, C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0033499   galactose catabolic process via UDP-galactose  
GO:0008270   zinc ion binding  
GO:0008108   UDP-glucose:hexose-1-phosphate uridylyltransferase activity  
GO:0003824   catalytic activity  
GO:0006012   galactose metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 180 1gup (A) 161 339 27.00 0 1 1.31118 0.05
5 179 6gqd (A) 181 361 29.00 0 1 1.27865 0.32
35 145 3o1c (A) 12 120 15.00 0 0.87 0.85206 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_129227)

Species Accession Gene Product
Arabidopsis thaliana AT5G18200   putative galactose-1-phosphate uridyltransferase
Candida albicans CaO19.3675   galactose-1-P uridylyl transferase
Candida albicans CaO19.11159   galactose-1-P uridylyl transferase
Caenorhabditis elegans CELE_ZK1058.3   Protein ZK1058.3
Drosophila melanogaster Dmel_CG9232   Galactose-1-phosphate uridylyltransferase
Escherichia coli b0758   galactose-1-phosphate uridylyltransferase
Echinococcus granulosus EgrG_000689300   galactose 1 phosphate uridylyltransferase
Echinococcus multilocularis EmuJ_000689300   galactose 1 phosphate uridylyltransferase
Homo sapiens ENSG00000213930   galactose-1-phosphate uridylyltransferase
Mus musculus 14430   galactose-1-phosphate uridyl transferase
Mycobacterium tuberculosis Rv0618   Probable galactose-1-phosphate uridylyltransferase GalTa [first part]
Mycobacterium tuberculosis Rv0619   Probable galactose-1-phosphate uridylyltransferase GalTb [second part]
Mycobacterium ulcerans MUL_0691   galactose-1-phosphate uridylyltransferase
Oryza sativa 4342514   Os07g0171200
Saccharomyces cerevisiae YBR018C   UDP-glucose:hexose-1-phosphate uridylyltransferase
Schistosoma japonicum Sjp_0056930   ko:K00965 UDPglucose--hexose-1-phosphate uridylyltransferase [EC2.7.7.12], putative
Schistosoma mansoni Smp_059880   galactose-1-phosphate uridylyltransferase
Schmidtea mediterranea mk4.001630.03   Probable galactose-1-phosphate uridylyltransferase

Essentiality

Rv0619 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu629 this record Mycobacterium tuberculosis non-essential nmpdr
b0758 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0619 (Mycobacterium tuberculosis), Probable galactose-1-phosphate uridylyltransferase GalTb [second part]
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