Detailed view for Rv0644c

Basic information

TDR Targets ID: 6469
Mycobacterium tuberculosis, Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase)
Source Database / ID:  Tuberculist 

pI: 5.724 | Length (AA): 287 | MW (Da): 32724 | Paralog Number: 4

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02353   Mycolic acid cyclopropane synthetase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008825   cyclopropane-fatty-acyl-phospholipid synthase activity  
GO:0008610   lipid biosynthetic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 287 1tpy (A) 3 287 99.99 0 1 2.26223 -1.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1KP9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1KPG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1KPH:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1KPI:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1L1E:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1TPY:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2FK7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2FK8:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3HA3:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3HA5:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3HA7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3HEM:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127897)

Species Accession Gene Product
Arabidopsis thaliana AT3G23470   cyclopropane-fatty-acyl-phospholipid synthase
Arabidopsis thaliana AT3G23480   Cyclopropane-fatty-acyl-phospholipid synthase
Arabidopsis thaliana AT3G23530   Cyclopropane-fatty-acyl-phospholipid synthase
Arabidopsis thaliana AT3G23510   cyclopropane-fatty-acyl-phospholipid synthase
Candida albicans CaO19.12294   potential cyclopropane-fatty-acyl-phospholipid synthase similar to plant cyclopropane synthase (AAM33848)
Candida albicans CaO19.4831   potential cyclopropane-fatty-acyl-phospholipid synthase similar to plant cyclopropane synthase (AAM33848)
Caenorhabditis elegans CELE_F13D12.9   Protein F13D12.9
Dictyostelium discoideum DDB_G0283847   cyclopropane fatty acid synthase
Dictyostelium discoideum DDB_G0267592   hypothetical protein
Escherichia coli b1661   cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase)
Leishmania braziliensis LbrM.08.0590   cyclopropane-fatty-acyl-phospholipid synthase
Leishmania donovani LdBPK_080560.1   cyclopropane-fatty-acyl-phospholipid syntahse
Leishmania infantum LinJ.08.0560   cyclopropane-fatty-acyl-phospholipid syntahse
Leishmania mexicana LmxM.08.0545   cyclopropane-fatty-acyl-phospholipid synthase
Loa Loa (eye worm) LOAG_13555   hypothetical protein
Loa Loa (eye worm) LOAG_14720   hypothetical protein
Loa Loa (eye worm) LOAG_13554   hypothetical protein
Mycobacterium leprae ML2334   POSSIBLE FATTY ACID SYNTHASE
Mycobacterium tuberculosis Rv3720   Possible fatty acid synthase
Mycobacterium tuberculosis Rv0447c   Probable cyclopropane-fatty-acyl-phospholipid synthase UfaA1 (cyclopropane fatty acid synthase) (CFA synthase)
Mycobacterium tuberculosis Rv0644c   Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase)
Mycobacterium tuberculosis Rv3392c   Cyclopropane-fatty-acyl-phospholipid synthase 1 CmaA1 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic ac
Mycobacterium tuberculosis Rv0449c   Conserved hypothetical protein
Mycobacterium ulcerans MUL_1404   oxidoreductase
Mycobacterium ulcerans MUL_0731   methoxy mycolic acid synthase 2, MmaA2
Mycobacterium ulcerans MUL_3772   methoxy mycolic acid synthase 5 MmaA5
Mycobacterium ulcerans MUL_1402   cyclopropane-fatty-acyl-phospholipid synthase
Oryza sativa 4351940   Os12g0267200
Oryza sativa 4343210   Os07g0474600
Onchocerca volvulus OVOC10396  

Essentiality

Rv0644c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3452 Mycobacterium tuberculosis non-essential nmpdr
mtu656 this record Mycobacterium tuberculosis non-essential nmpdr
mtu3784 Mycobacterium tuberculosis non-essential nmpdr
mtu450 Mycobacterium tuberculosis non-essential nmpdr
b1661 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

63 literature references were collected for this gene.

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Gene identifier Rv0644c (Mycobacterium tuberculosis), Methoxy mycolic acid synthase 2 MmaA2 (methyl mycolic acid synthase 2) (MMA2) (hydroxy mycolic acid synthase)
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