Detailed view for Rv0685

Basic information

TDR Targets ID: 981710
Mycobacterium tuberculosis, Probable iron-regulated elongation factor TU Tuf (EF-TU)
Source Database / ID:  Tuberculist 

pI: 5.1195 | Length (AA): 396 | MW (Da): 43562 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 395 3avx (A) 279 677 72.00 0 1 1.73557 -0.27
8 395 5w7q (A) 8 393 78.00 0 1 1.892 -0.93
9 396 5w75 (A) 9 394 69.00 0 1 1.854 -1.05

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126954)

Species Accession Gene Product
Arabidopsis thaliana AT4G20360   RAB GTPase homolog E1b
Arabidopsis thaliana AT4G02930   putative elongation factor Tu
Babesia bovis BBOV_III001500   translation elongation factor Tu
Babesia bovis BBOV_V000220   EFTU_STAAS Elongation factor Tu, putative
Brugia malayi Bm1_54320   elongation factor Tu homologue precursor
Candida albicans CaO19.13468   translation elongation factor Tu
Candida albicans CaO19.6047   translation elongation factor Tu
Caenorhabditis elegans CELE_Y71H2AM.23   Protein TUFM-1
Chlamydia trachomatis CT_322   elongation factor Tu
Dictyostelium discoideum DDB_G0289593   elongation factor Tu domain-containing protein
Drosophila melanogaster Dmel_CG6050   Elongation factor Tu mitochondrial
Escherichia coli b3980   translation elongation factor EF-Tu 2
Escherichia coli b3339   translation elongation factor EF-Tu 1
Echinococcus granulosus EgrG_000102600   elongation factor tu ef tu
Echinococcus multilocularis EmuJ_000102600   elongation factor tu (ef tu)
Homo sapiens ENSG00000178952   Tu translation elongation factor, mitochondrial
Leishmania braziliensis LbrM.18.0830   elongation factor Tu, putative
Leishmania donovani LdBPK_180740.1   Elongation factor Tu, mitochondrial, putative
Leishmania infantum LinJ.18.0740   elongation factor Tu, putative
Leishmania major LmjF.18.0740   elongation factor Tu, putative
Leishmania mexicana LmxM.18.0740   elongation factor Tu, putative
Loa Loa (eye worm) LOAG_10636   elongation factor Tu1
Loa Loa (eye worm) LOAG_11346   elongation factor Tu
Mycobacterium leprae ML1877c   PROBABLE IRON-REGULATED ELONGATION FACTOR TU TUF (EF-TU)
Mus musculus ENSMUSG00000073838   Tu translation elongation factor, mitochondrial
Mycobacterium tuberculosis Rv0685   Probable iron-regulated elongation factor TU Tuf (EF-TU)
Mycobacterium ulcerans MUL_0766   elongation factor Tu
Mycobacterium ulcerans MUL_2857   iron-regulated elongation factor Tu Tuf-like
Neospora caninum NCLIV_025450   elongation factor Tu, putative
Oryza sativa 9270837   Os02g0595700
Oryza sativa 4334800   Os03g0851100
Plasmodium berghei PBANKA_1345600   elongation factor Tu, putative
Plasmodium berghei PBANKA_API00280   elongation factor Tu, putative
Plasmodium falciparum PF3D7_1330600   elongation factor Tu, putative
Plasmodium falciparum PF3D7_API02900   elongation factor Tu
Plasmodium knowlesi PKNH_1269800   elongation factor Tu, putative
Plasmodium knowlesi PKNH_API03800   elongation factor Tu, putative
Plasmodium vivax PVX_082470   elongation factor Tu, mitochondrial precursor, putative
Plasmodium yoelii PY05361   translation elongation factor Tu
Plasmodium yoelii PY06134   translation elongation factor Tu
Saccharomyces cerevisiae YOR187W   translation elongation factor Tu
Schistosoma japonicum Sjp_0215290   ko:K02358 elongation factor EF-Tu, putative
Schistosoma mansoni Smp_073500.1   elongation factor tu (ef-tu)
Schistosoma mansoni Smp_073500.2   elongation factor tu (ef-tu)
Schmidtea mediterranea mk4.012288.00   Elongation factor Tu, mitochondrial
Schmidtea mediterranea mk4.002140.01  
Schmidtea mediterranea mk4.008229.00   Elongation factor Tu, mitochondrial
Trypanosoma brucei gambiense Tbg972.10.16140   elongation factor Tu, putative
Trypanosoma brucei Tb927.10.13360   Elongation factor Tu, mitochondrial
Trypanosoma congolense TcIL3000_10_11450   Elongation factor Tu, mitochondrial, putative
Trypanosoma cruzi TcCLB.506357.40   Elongation factor Tu, mitochondrial, putative
Trypanosoma cruzi TcCLB.511277.70   Elongation factor Tu, mitochondrial, putative
Toxoplasma gondii TGME49_302060   elongation factor tu, apicoplast, putative
Toxoplasma gondii TGME49_262380   elongation factor Tu, putative
Toxoplasma gondii TGME49_302050   elongation factor tu, apicoplast, putative
Treponema pallidum TP0187   elongation factor Tu
Theileria parva TP05_0019   elongation factor TU, putative
Theileria parva TP03_0698   elongation factor Tu, putative
Wolbachia endosymbiont of Brugia malayi Wbm0343   elongation factor Tu
Wolbachia endosymbiont of Brugia malayi Wbm0653   elongation factor Tu

Essentiality

Rv0685 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.13360 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.13360 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.13360 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3339 Escherichia coli non-essential goodall
b3980 Escherichia coli non-essential goodall
CELE_Y71H2AM.23 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y71H2AM.23 Caenorhabditis elegans larval arrest wormbase
CELE_Y71H2AM.23 Caenorhabditis elegans sterile wormbase
PBANKA_1345600 Plasmodium berghei Essential plasmo
TGME49_262380 Toxoplasma gondii Probably essential sidik
TGME49_302050 Toxoplasma gondii Probably essential sidik
TGME49_302060 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv0685 (Mycobacterium tuberculosis), Probable iron-regulated elongation factor TU Tuf (EF-TU)
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