Detailed view for Rv0727c

Basic information

TDR Targets ID: 6105
Mycobacterium tuberculosis, Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase)
Source Database / ID:  Tuberculist 

pI: 5.8585 | Length (AA): 218 | MW (Da): 23066 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00596   Class II Aldolase and Adducin N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 216 1k0w (A) 1 223 23.00 0.0000062 1 1.23565 -0.16
5 210 1e4c (P) 4 206 27.00 0.0000063 1 1.35195 -0.69
11 205 6btd (A) 10 202 35.00 0 1 1.4046 -0.86
11 204 4c24 (A) 8 199 36.00 0 1 1.39831 -0.84

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127052)

Species Accession Gene Product
Brugia malayi Bm1_54595   Adducin-related protein 1
Candida albicans CaO19.6679   similar to bacterial aldolase
Candida albicans CaO19.4789   similar to bacterial aldolase
Candida albicans CaO19.12253   similar to bacterial aldolase
Caenorhabditis elegans CELE_F57F5.4   Protein ADD-2, isoform B
Caenorhabditis elegans CELE_F39C12.2   Protein ADD-1, isoform A
Dictyostelium discoideum DDB_G0282009   alpha adducin
Escherichia coli b2738   putative class II aldolase
Escherichia coli b2800   L-fuculose-1-phosphate aldolase
Echinococcus granulosus EgrG_000830600   adducin related protein
Echinococcus granulosus EgrG_000240700   nervous system adducin
Echinococcus granulosus EgrG_000104300   alpha adducin
Echinococcus multilocularis EmuJ_000104300   alpha adducin
Echinococcus multilocularis EmuJ_000240700   nervous system adducin
Echinococcus multilocularis EmuJ_000830600   adducin related protein
Homo sapiens ENSG00000075340   adducin 2 (beta)
Homo sapiens ENSG00000087274   adducin 1 (alpha)
Homo sapiens ENSG00000148700   adducin 3 (gamma)
Loa Loa (eye worm) LOAG_16242   hypothetical protein
Loa Loa (eye worm) LOAG_10542   hypothetical protein
Loa Loa (eye worm) LOAG_15304   hypothetical protein
Mus musculus ENSMUSG00000025026   adducin 3 (gamma)
Mus musculus ENSMUSG00000029106   adducin 1 (alpha)
Mus musculus 11519   adducin 2 (beta)
Mycobacterium tuberculosis Rv0727c   Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase)
Mycobacterium ulcerans MUL_3144   ribulose-5-phosphate 4-epimerase, AraD
Mycobacterium ulcerans MUL_0822   L-fuculose phosphate aldolase
Schistosoma japonicum Sjp_0306220   Conserved hypothetical protein
Schistosoma japonicum Sjp_0030590   Alpha-adducin, putative
Schistosoma japonicum Sjp_0217310   Alpha-adducin, putative
Schistosoma japonicum Sjp_0054700   Protein hu-li tai shao, putative
Schistosoma japonicum Sjp_0024050   Alpha-adducin, putative
Schistosoma japonicum Sjp_0054710   Alpha-adducin, putative
Schistosoma mansoni Smp_083040.1   adducin related protein
Schistosoma mansoni Smp_014670.2   adducin related protein
Schistosoma mansoni Smp_083040.2   adducin related protein
Schistosoma mansoni Smp_071140   adducin related protein
Schistosoma mansoni Smp_014670.1   adducin related protein
Schmidtea mediterranea mk4.022678.00   Adducin-related protein 2
Schmidtea mediterranea mk4.022272.01  
Schmidtea mediterranea mk4.001426.04   Adducin-related protein 2
Schmidtea mediterranea mk4.000957.00   Adducin-related protein 1
Schmidtea mediterranea mk4.000741.05   Adducin-related protein 1
Schmidtea mediterranea mk4.019881.00   Adducin-related protein 1
Schmidtea mediterranea mk4.017333.01  
Schmidtea mediterranea mk4.007899.00   Adducin-related protein 2
Schmidtea mediterranea mk4.014081.00  
Schmidtea mediterranea mk4.001959.01   Adducin-related protein 1
Schmidtea mediterranea mk4.022272.00   Adducin-related protein 1

Essentiality

Rv0727c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu740 this record Mycobacterium tuberculosis non-essential nmpdr
b2738 Escherichia coli non-essential goodall
b2800 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv0727c (Mycobacterium tuberculosis), Possible L-fuculose phosphate aldolase FucA (L-fuculose-1-phosphate aldolase)
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