Detailed view for Rv0753c

Basic information

TDR Targets ID: 6492
Mycobacterium tuberculosis, Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH)
Source Database / ID:  Tuberculist 

pI: 5.4488 | Length (AA): 510 | MW (Da): 54454 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00171   Aldehyde dehydrogenase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016620   oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor  
GO:0004491   methylmalonate-semialdehyde dehydrogenase (acylating) activity  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006573   valine metabolic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 506 5tjr (A) 2 494 57.00 0 1 1.60434 -0.56
4 500 4iym (A) 1 486 68.00 0 1 1.84711 -1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_128625)

Species Accession Gene Product
Arabidopsis thaliana AT2G14170   Methylmalonate-semialdehyde dehydrogenase [acylating]
Candida albicans CaO19.8361   cytosolic acetaldehyde dehydrogenase
Candida albicans CaO19.742   cytosolic acetaldehyde dehydrogenase
Caenorhabditis elegans CELE_F13D12.4   Protein ALH-8, isoform B
Dictyostelium discoideum DDB_G0289085   methylmalonate-semialdehyde dehydrogenase
Drosophila melanogaster Dmel_CG17896   CG17896 gene product from transcript CG17896-RB
Homo sapiens ENSG00000119711   aldehyde dehydrogenase 6 family, member A1
Mus musculus ENSMUSG00000021238   aldehyde dehydrogenase family 6, subfamily A1
Mycobacterium tuberculosis Rv0753c   Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH)
Mycobacterium ulcerans MUL_0837   methylmalonate semialdehyde dehydrogenase, MmsA
Neospora caninum NCLIV_055270   Aldehyde dehydrogenase, related
Oryza sativa 4342610   Os07g0188800
Schmidtea mediterranea mk4.064011.00   Probable methylmalonate-semialdehyde dehydrogenase
Schmidtea mediterranea mk4.000829.00   Probable methylmalonate-semialdehyde dehydrogenase
Toxoplasma gondii TGME49_311370   methylmalonate-semialdehyde dehydrogenase [acylating], putative

Essentiality

Rv0753c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu767 this record Mycobacterium tuberculosis non-essential nmpdr
TGME49_311370 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Aldehyde dehydrogenase 1A1 501 aa 31.0% 500 aa Compounds References
Rattus norvegicus Aldehyde dehydrogenase, cytosolic 1 501 aa 30.7% 499 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0753c (Mycobacterium tuberculosis), Probable methylmalonate-semialdehyde dehydrogenase MmsA (methylmalonic acid semialdehyde dehydrogenase) (MMSDH)
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